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Literature summary for 4.2.3.30 extracted from

  • Bromann, K.; Toivari, M.; Viljanen, K.; Ruohonen, L.; Nakari-Setaelae, T.
    Engineering Aspergillus nidulans for heterologous ent-kaurene and gamma-terpinene production (2016), Appl. Microbiol. Biotechnol., 100, 6345-6359 .
    View publication on PubMed
Search for more literature for 4.2.3.30

Cloned(Commentary)

Cloned (Comment) Organism
gene AN1594, the AN1594 overexpression strain A772 oe:AN1594 is created by placing AN1594 under the control of the constitutively active gpdA promoter in Aspergillus nidulans strain A772, quantitative RT-PCR enzyme expression analysis Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Aspergillus nidulans
-
 ent-pimara-8(14),15-diene + diphosphate
-
 ?
ent-copalyl diphosphate Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139
-
 ent-pimara-8(14),15-diene + diphosphate
-
 ?
ent-copalyl diphosphate Aspergillus nidulans A4
-
 ent-pimara-8(14),15-diene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans Q5BCY6
-
-
Aspergillus nidulans Q5BCY6 i.e. Emericella nidulans
-
Aspergillus nidulans A4 Q5BCY6
-
-
Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 Q5BCY6 i.e. Emericella nidulans
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Aspergillus nidulans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
 Aspergillus nidulans ent-pimara-8(14),15-diene + diphosphate
-
 ?
ent-copalyl diphosphate
-
 Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 ent-pimara-8(14),15-diene + diphosphate
-
 ?
ent-copalyl diphosphate
-
 Aspergillus nidulans A4 ent-pimara-8(14),15-diene + diphosphate
-
 ?
additional information GC/MS analysis of enzyme products Aspergillus nidulans ?
-
 ?
additional information GC/MS analysis of enzyme products Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 ?
-
 ?
additional information GC/MS analysis of enzyme products Aspergillus nidulans A4 ?
-
 ?

Synonyms

Synonyms Comment Organism
AN1594 locus name Aspergillus nidulans
ANIA_01594 gene name, UniProt Aspergillus nidulans
ent-pimara-8(14),15-diene synthase
-
 Aspergillus nidulans
pbcA
-
 Aspergillus nidulans
PD synthase
-
 Aspergillus nidulans

Expression

Organism Comment Expression
Aspergillus nidulans wild-type Aspergillus nidulans strain overexpressing the cluster-specific transcription factor, pbcR, shows upregulation of the cluster genes leading to ent-pimara-8(14),15-diene production in Aspergillus nidulans and 11.7fold increased expression of gene ANIA_01594 up