Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D71N | 2500fold decrease in kcat | Escherichia coli |
General Stability | Organism |
---|---|
D71N mutant enzyme rapidly becomes inactive in the absence of phosphate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphoglycolohydroxamic acid | tight binding inhibitor, neither a strictly competitive, non-competitive, nor uncompetitive mechanism | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
glycerone phosphate = 2-oxopropanal + phosphate | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate | converts dihydrocyacetone phosphate to a cis-enediolic intermediate, then catalyses the elimination of phosphate to form the enol of methylglyoxal | Escherichia coli | methylglyoxal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | homohexamer, crystallization experiments | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000093 | - |
phosphoglycolohydroxamic acid | - |
Escherichia coli |