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Literature summary for 4.2.3.3 extracted from

  • Marks, G.T.; Harris, T.K.; Massiah, M.A.; Mildvan, A.S.; Harrison, D.H.
    Mechanistic implications of methylglyoxal synthase complexed with phosphoglycolohydroxamic acid as observed by X-ray crystallography and NMR spectroscopy (2001), Biochemistry, 40, 6805-6818.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method Escherichia coli

Protein Variants

Protein Variants Comment Organism
D71N 2500fold decrease in kcat Escherichia coli

General Stability

General Stability Organism
D71N mutant enzyme rapidly becomes inactive in the absence of phosphate Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
phosphoglycolohydroxamic acid tight binding inhibitor, neither a strictly competitive, non-competitive, nor uncompetitive mechanism Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
glycerone phosphate = 2-oxopropanal + phosphate mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate converts dihydrocyacetone phosphate to a cis-enediolic intermediate, then catalyses the elimination of phosphate to form the enol of methylglyoxal Escherichia coli methylglyoxal + phosphate
-
?

Subunits

Subunits Comment Organism
hexamer homohexamer, crystallization experiments Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000093
-
phosphoglycolohydroxamic acid
-
Escherichia coli