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Literature summary for 4.2.3.3 extracted from
- Characterization of methylglyoxal synthase from Clostridium acetobutylicum ATCC 824 and its use in the formation of 1,2-propanediol (1999), Appl. Environ. Microbiol., 65, 3244-3247.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Clostridium acetobutylicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Clostridium acetobutylicum |  |
| ATP | - |
Clostridium acetobutylicum | |
| diphosphate | - |
Clostridium acetobutylicum | |
| fructose 1,6-diphosphate | - |
Clostridium acetobutylicum | |
| phosphate | - |
Clostridium acetobutylicum | |
| phosphoenolpyruvate | - |
Clostridium acetobutylicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.53 | - |
dihydroxyacetone phosphate | - |
Clostridium acetobutylicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
4 * 15000, SDS-PAGE | Clostridium acetobutylicum |
| 60000 | - |
gel filtration | Clostridium acetobutylicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
ATCC 824 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Clostridium acetobutylicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 1 month | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroxyacetone phosphate | - |
Clostridium acetobutylicum | methylglyoxal + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 15000, SDS-PAGE | Clostridium acetobutylicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Clostridium acetobutylicum |
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Release 2023.1 (January 2023)
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