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Literature summary for 4.2.3.29 extracted from

  • Morrone, D.; Hillwig, M.L.; Mead, M.E.; Lowry, L.; Fulton, D.B.; Peters, R.J.
    Evident and latent plasticity across the rice diterpene synthase family with potential implications for the evolution of diterpenoid metabolism in the cereals (2011), Biochem. J., 435, 589-595.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.29

Cloned(Commentary)

Cloned (Comment) Organism
expression as glutathione-S-transferase fusion protein Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM value for substrate syn-copalyl diphosphate is 0.005 mM Oryza sativa
0.0006
-
 ent-copalyl diphosphate 22°C, pH not specified in the publication Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q2QQJ5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
 Oryza sativa ent-pimara-8(14),15-diene + diphosphate
-
 ?
additional information additionally converts syn-copalyl diphosphate into syn-labda-8(17),12E,14-triene Oryza sativa ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information Kcat value for substrate syn-copalyl diphosphate is 0.00083 per s Oryza sativa
0.00033
-
 ent-copalyl diphosphate 22°C, pH not specified in the publication Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information Kcat/KM value for substrate syn-copalyl diphosphate is 0.17 per s and mM Oryza sativa
0.55
-
 ent-copalyl diphosphate 22°C, pH not specified in the publication Oryza sativa