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Literature summary for 4.2.3.27 extracted from
- Biochemical properties of isoprene synthase in poplar (Populus x canescens) (2005), Planta, 222, 777-786.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ispS, expression of N-terminally or C-terminally His-tagged enzyme without plastidial targeting sequence in Escherichia coli strain TG1 | Populus tremula x Populus alba |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by PMSF | Populus tremula x Populus alba |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
dimethylallyl diphosphate | pH 7.8, 40°C, recombinant C-terminally His-tagged enzyme | Populus tremula x Populus alba |  |
| 2.45 | - |
dimethylallyl diphosphate | pH 7.8, 40°C, native enzyme | Populus tremula x Populus alba | |
| 3.1 | - |
dimethylallyl diphosphate | pH 7.8, 40°C, recombinant N-terminally His-tagged enzyme | Populus tremula x Populus alba | |
| 3.68 | - |
dimethylallyl diphosphate | pH 7.8, 40°C, recombinant untagged enzyme | Populus tremula x Populus alba | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | the enzyme is localized in the stroma and is also attached to the stromal side of the thylkaoid membrane | Populus tremula x Populus alba | 9507 | - |
| additional information | subcellular localization study, overview | Populus tremula x Populus alba | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, optimal at 20 mM | Populus tremula x Populus alba | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
native enzyme, gel filtration | Populus tremula x Populus alba |
| 53000 | - |
recombinant untagged enzyme, gel filtration | Populus tremula x Populus alba |
| 62000 | - |
recombinant untagged enzyme, native PAGE | Populus tremula x Populus alba |
| 68000 | - |
1 * 68000, C-terminally His-tagged enzyme, SDS-PAGE, 1 * 78000, N-terminally His-tagged enzyme, SDS-PAGE | Populus tremula x Populus alba |
| 78000 | - |
1 * 68000, C-terminally His-tagged enzyme, SDS-PAGE, 1 * 78000, N-terminally His-tagged enzyme, SDS-PAGE | Populus tremula x Populus alba |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | Populus tremula x Populus alba | substrate limitations can be responsible for great variations of enzyme activity from leaf to leaf | isoprene + diphosphate | isoprene is a volatile product emmitted to the atmosphere | ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus tremula x Populus alba | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially from leaves, recombinant His-tagged enzyme without plastidial targeting sequence from Escherichia coli strain TG1 by nickel affinity chromatography | Populus tremula x Populus alba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Populus tremula x Populus alba | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | - |
Populus tremula x Populus alba | isoprene + diphosphate | i.e. 2-methyl-1,3-butadiene | ir | |
| dimethylallyl diphosphate | substrate limitations can be responsible for great variations of enzyme activity from leaf to leaf | Populus tremula x Populus alba | isoprene + diphosphate | isoprene is a volatile product emmitted to the atmosphere | ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 68000, C-terminally His-tagged enzyme, SDS-PAGE, 1 * 78000, N-terminally His-tagged enzyme, SDS-PAGE | Populus tremula x Populus alba |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ISPS | - |
Populus tremula x Populus alba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant N-terminally His-tagged enzyme, recombinant untagged enzyme, and native enzyme | Populus tremula x Populus alba |
| 40 | 50 | recombinant C-terminally His-tagged enzyme | Populus tremula x Populus alba |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 55 | recombinant N-terminally His-tagged enzyme, recombinant untagged enzyme, and native enzyme | Populus tremula x Populus alba |
| 40 | 60 | recombinant C-terminally His-tagged enzyme | Populus tremula x Populus alba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.5 | recombinant C-terminally His-tagged enzyme, recombinant untagged enzyme, and native enzyme | Populus tremula x Populus alba |
| 9 | - |
recombinant N-terminally His-tagged enzyme | Populus tremula x Populus alba |
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