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Literature summary for 4.2.3.25 extracted from

  • Karuppiah, V.; Ranaghan, K.E.; Leferink, N.G.H.; Johannissen, L.O.; Shanmugam, M.; Ni Cheallaigh, A.; Bennett, N.J.; Kearsey, L.J.; Takano, E.; Gardiner, J.M.; van der Kamp, M.W.; Hay, S.; Mulholland, A.J.; Leys, D.; Scrutton, N.S.
    Structural basis of catalysis in the bacterial monoterpene synthases linalool synthase and 1,8-cineole synthase (2017), ACS Catal., 7, 6268-6282 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ArcticExpress (DE3) cells Streptomyces clavuligerus

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method, using 40% v/v glycerol and 20% w/v PEG 4000 Streptomyces clavuligerus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ contains 3 Mg2+ ions Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + H2O Streptomyces clavuligerus
-
(3S)-linalool + diphosphate
-
?
geranyl diphosphate + H2O Streptomyces clavuligerus ATCC 27064
-
(3S)-linalool + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
-
-
-
Streptomyces clavuligerus ATCC 27064
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex S75 gel filtration Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
-
Streptomyces clavuligerus trans-nerolidol + diphosphate
-
?
(2E,6E)-farnesyl diphosphate + H2O
-
Streptomyces clavuligerus ATCC 27064 trans-nerolidol + diphosphate
-
?
2-fluorogeranyl diphosphate + H2O
-
Streptomyces clavuligerus ?
-
?
2-fluorogeranyl diphosphate + H2O
-
Streptomyces clavuligerus ATCC 27064 ?
-
?
2-fluoroneryl diphosphate + H2O
-
Streptomyces clavuligerus ?
-
?
2-fluoroneryl diphosphate + H2O
-
Streptomyces clavuligerus ATCC 27064 ?
-
?
geranyl diphosphate + H2O
-
Streptomyces clavuligerus (3S)-linalool + diphosphate
-
?
geranyl diphosphate + H2O
-
Streptomyces clavuligerus ATCC 27064 (3S)-linalool + diphosphate
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Streptomyces clavuligerus

Synonyms

Synonyms Comment Organism
bLINS
-
Streptomyces clavuligerus
linalool synthase
-
Streptomyces clavuligerus