Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ArcticExpress (DE3) cells | Streptomyces clavuligerus |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method, using 40% v/v glycerol and 20% w/v PEG 4000 | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | contains 3 Mg2+ ions | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + H2O | Streptomyces clavuligerus | - |
(3S)-linalool + diphosphate | - |
? | |
geranyl diphosphate + H2O | Streptomyces clavuligerus ATCC 27064 | - |
(3S)-linalool + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
- |
- |
Streptomyces clavuligerus ATCC 27064 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex S75 gel filtration | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | - |
Streptomyces clavuligerus | trans-nerolidol + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + H2O | - |
Streptomyces clavuligerus ATCC 27064 | trans-nerolidol + diphosphate | - |
? | |
2-fluorogeranyl diphosphate + H2O | - |
Streptomyces clavuligerus | ? | - |
? | |
2-fluorogeranyl diphosphate + H2O | - |
Streptomyces clavuligerus ATCC 27064 | ? | - |
? | |
2-fluoroneryl diphosphate + H2O | - |
Streptomyces clavuligerus | ? | - |
? | |
2-fluoroneryl diphosphate + H2O | - |
Streptomyces clavuligerus ATCC 27064 | ? | - |
? | |
geranyl diphosphate + H2O | - |
Streptomyces clavuligerus | (3S)-linalool + diphosphate | - |
? | |
geranyl diphosphate + H2O | - |
Streptomyces clavuligerus ATCC 27064 | (3S)-linalool + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Streptomyces clavuligerus |
Synonyms | Comment | Organism |
---|---|---|
bLINS | - |
Streptomyces clavuligerus |
linalool synthase | - |
Streptomyces clavuligerus |