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Literature summary for 4.2.3.25 extracted from
- Characterisation of an (S)-linalool synthase from kiwifruit (Actinidia arguta) that catalyses the first committed step in the production of floral lilac compounds (2010), Funct. Plant Biol., 37, 232-243.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient expression in Nicotiana benthamiana, His-tagged version expressed in Escherichia coli | Actinidia arguta |
| transient expression in Nicotiana benthamiana, His-tagged version expressed in Escherichia coli | Actinidia polygama |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
geranyl diphosphate | 25 mM Mg2+ | Actinidia arguta |  |
| 0.0026 | - |
geranyl diphosphate | 25 mM Mg2+ | Actinidia polygama | |
| 0.0266 | - |
Mn2+ | cofactor Mn2+, substrate 0.05 mM geranyl diphosphate | Actinidia polygama | |
| 0.0282 | - |
Mn2+ | cofactor Mn2+, substrate 0.05 mM geranyl diphosphate | Actinidia arguta | |
| 0.346 | - |
Mg2+ | cofactor Mg2+, substrate 0.05 mM geranyl diphosphate | Actinidia arguta | |
| 0.891 | - |
Mg2+ | cofactor Mg2+, substrate 0.05 mM geranyl diphosphate | Actinidia polygama | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ or Mn2+ required | Actinidia arguta | |
| Mg2+ | Mg2+ or Mn2+ required | Actinidia polygama | |
| Mn2+ | Mg2+ or Mn2+ required | Actinidia arguta | |
| Mn2+ | Mg2+ or Mn2+ required | Actinidia polygama | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE | Actinidia arguta |
| 65000 | - |
x * 65000, SDS-PAGE | Actinidia polygama |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinidia arguta | D4N3A0 | - |
- |
| Actinidia polygama | D4N3A1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ affinity chromatography, gel filtration | Actinidia arguta |
| Ni2+ affinity chromatography, gel filtration | Actinidia polygama |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Actinidia polygama | - |
| petal | - |
Actinidia arguta | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.013 | - |
in the presence of Mg2+, 22% activity in the presence of Mn2+ | Actinidia polygama |
| 0.039 | - |
in the presence of Mg2+, 50% activity in the presence of Mn2+ | Actinidia arguta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate + H2O | - |
Actinidia arguta | (3S)-linalool + diphosphate | - |
? | |
| geranyl diphosphate + H2O | - |
Actinidia polygama | (3S)-linalool + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Actinidia arguta |
| ? | x * 65000, SDS-PAGE | Actinidia polygama |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (S)-linalool synthase | - |
Actinidia arguta |
| (S)-linalool synthase | - |
Actinidia polygama |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
geranyl diphosphate | - |
Actinidia polygama | |
| 0.044 | - |
geranyl diphosphate | - |
Actinidia arguta | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.6 | - |
geranyl diphosphate | - |
Actinidia polygama | |
| 21 | - |
geranyl diphosphate | - |
Actinidia arguta | |
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