Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | required for stability | Clarkia breweri |
General Stability | Organism |
---|---|
DTT is required for enzyme stability, in absence of DTT the purified enzyme loses 95% of its activity within 2 h at 4°C | Clarkia breweri |
hexane and pentane stabilize the enzyme | Clarkia breweri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no effects by 0.1% w/v Triton X-100 | Clarkia breweri | |
SDS | leads to complete loss of activity at about 0.1% | Clarkia breweri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
geranyl diphosphate | pH 7.4, 20°C | Clarkia breweri | |
0.045 | - |
Mn2+ | cofactor Mn2+, pH 7.4, 20°C | Clarkia breweri | |
0.33 | - |
Mg2+ | cofactor Mg2+, pH 7.4, 20°C | Clarkia breweri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | half as effective as Mn2+ | Clarkia breweri | |
Mn2+ | absolutely required for activity, preferred divalent cation | Clarkia breweri | |
additional information | no activity with Cu2+, Zn2+, Ca2+, Co2+ | Clarkia breweri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
gel filtration | Clarkia breweri |
79000 | - |
1 * 79000, SDS-PAGE | Clarkia breweri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + H2O | Clarkia breweri | - |
(3S)-linalool + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clarkia breweri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 26fold from stigmata of freshly opened flowers by anion exchange and hydroxyapatite chromatography to over 95% purity | Clarkia breweri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
geranyl diphosphate + H2O = (3S)-linalool + diphosphate | reaction mechanism | Clarkia breweri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stigma | - |
Clarkia breweri | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0004 | - |
purified enzyme | Clarkia breweri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-linalyl diphosphate + H2O | low activity, 15% of the maximal activity with geranyl diphosphate | Clarkia breweri | (3S)-linalool + diphosphate | - |
? | |
geranyl diphosphate + H2O | - |
Clarkia breweri | (3S)-linalool + diphosphate | - |
? | |
geranyl diphosphate + H2O | enantiospecific reaction | Clarkia breweri | (3S)-linalool + diphosphate | product identification | ? | |
additional information | no activity with (R)-linalyl diphosphate | Clarkia breweri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 79000, SDS-PAGE | Clarkia breweri |
Synonyms | Comment | Organism |
---|---|---|
LIS | - |
Clarkia breweri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Clarkia breweri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
broad optimum | Clarkia breweri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 8.4 | 80% of maximal activity within this range | Clarkia breweri |