Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Artemisia annua |
Protein Variants | Comment | Organism |
---|---|---|
G400A | the mutant accumulates more alpha-bisabolol compared to the wild type enzyme | Artemisia annua |
G439A | the mutant accumulates more alpha-bisabolol compared to the wild type enzyme | Artemisia annua |
G439A | the mutant displays 3-4fold increase in activity compared to the wild type enzyme | Artemisia annua |
H392A | the mutant displays 3-4fold increase in activity compared to the wild type enzyme | Artemisia annua |
H392I | the mutant displays 3-4fold increase in activity compared to the wild type enzyme | Artemisia annua |
H392N | the mutant displays 3-4fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448A | the mutant shows about 4fold increase in catalytic efficiency compared to the wild type enzyme | Artemisia annua |
H448C | the mutant displays 4fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448F | the mutant displays 4fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448G | the mutant displays 2fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448M | the mutant displays 2fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448N | the mutant displays 3fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448Q | the mutant displays 3fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448R | the mutant displays 2fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448S | the mutant displays 3fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448T | the mutant displays 2fold increase in activity compared to the wild type enzyme | Artemisia annua |
H448V | the mutant displays 2fold increase in activity compared to the wild type enzyme | Artemisia annua |
K449M | the mutant displays 3-4fold increase in activity compared to the wild type enzyme | Artemisia annua |
L515A | the mutant accumulates more alpha-bisabolol compared to the wild type enzyme | Artemisia annua |
L515C | the mutant accumulates more alpha-bisabolol compared to the wild type enzyme | Artemisia annua |
L515G | the mutant accumulates more alpha-bisabolol compared to the wild type enzyme | Artemisia annua |
L515S | the mutant accumulates more alpha-bisabolol compared to the wild type enzyme | Artemisia annua |
L515T | the mutant accumulates more alpha-bisabolol compared to the wild type enzyme | Artemisia annua |
Q518A | the mutant displays 3-4fold increase in activity compared to the wild type enzyme | Artemisia annua |
Q518L | the mutant displays 3-4fold increase in activity compared to the wild type enzyme | Artemisia annua |
T296A | the mutant retains its cyclization ability producing amorpha-4,11-diene | Artemisia annua |
T296L | the mutant loses its cyclization ability producing the acyclic beta-farnesene instead of amorpha-4,11-diene | Artemisia annua |
T296S | the mutant retains its cyclization ability producing amorpha-4,11-diene | Artemisia annua |
T296V | the mutant loses its cyclization ability producing the acyclic beta-farnesene instead of amorpha-4,11-diene | Artemisia annua |
T296Y | the mutant loses its cyclization ability producing the acyclic beta-farnesene instead of amorpha-4,11-diene | Artemisia annua |
T399A | the mutant exhibits an increase in the level of amorpha-4,7-diene | Artemisia annua |
T399E | inactive | Artemisia annua |
T399F | inactive | Artemisia annua |
T399G | the mutant exhibits an increase in the level of amorpha-4,7-diene | Artemisia annua |
T399I | the mutant exhibits an increase in the level of amorpha-4,7-diene | Artemisia annua |
T399K | inactive | Artemisia annua |
T399M | inactive | Artemisia annua |
T399N | the mutant exhibits an increase in the level of amorpha-4,7-diene | Artemisia annua |
T399P | inactive | Artemisia annua |
T399Q | inactive | Artemisia annua |
T399R | inactive | Artemisia annua |
T399S/H448A | the mutations improve the kcat value by 5times but at the expense of a raised Km making its catalytic efficiency about 3 times higher compared to the wild type enzyme | Artemisia annua |
T399V | the mutant exhibits an increase in the level of amorpha-4,7-diene | Artemisia annua |
V396A | the mutant displays 3-4fold increase in activity compared to the wild type enzyme | Artemisia annua |
V396I | the mutant displays 3-4fold increase in activity compared to the wild type enzyme | Artemisia annua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Artemisia annua | - |
amorpha-4,11-diene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemisia annua | Q9AR04 | - |
- |
Purification (Comment) | Organism |
---|---|
His MultiTrap column chromatography | Artemisia annua |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Artemisia annua | amorpha-4,11-diene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADS | - |
Artemisia annua |