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Literature summary for 4.2.3.24 extracted from

  • Abdallah, I.I.; van Merkerk, R.; Klumpenaar, E.; Quax, W.J.
    Catalysis of amorpha-4,11-diene synthase unraveled and improved by mutability landscape guided engineering (2018), Sci. Rep., 8, 9961 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Artemisia annua

Protein Variants

Protein Variants Comment Organism
G400A the mutant accumulates more alpha-bisabolol compared to the wild type enzyme Artemisia annua
G439A the mutant accumulates more alpha-bisabolol compared to the wild type enzyme Artemisia annua
G439A the mutant displays 3-4fold increase in activity compared to the wild type enzyme Artemisia annua
H392A the mutant displays 3-4fold increase in activity compared to the wild type enzyme Artemisia annua
H392I the mutant displays 3-4fold increase in activity compared to the wild type enzyme Artemisia annua
H392N the mutant displays 3-4fold increase in activity compared to the wild type enzyme Artemisia annua
H448A the mutant shows about 4fold increase in catalytic efficiency compared to the wild type enzyme Artemisia annua
H448C the mutant displays 4fold increase in activity compared to the wild type enzyme Artemisia annua
H448F the mutant displays 4fold increase in activity compared to the wild type enzyme Artemisia annua
H448G the mutant displays 2fold increase in activity compared to the wild type enzyme Artemisia annua
H448M the mutant displays 2fold increase in activity compared to the wild type enzyme Artemisia annua
H448N the mutant displays 3fold increase in activity compared to the wild type enzyme Artemisia annua
H448Q the mutant displays 3fold increase in activity compared to the wild type enzyme Artemisia annua
H448R the mutant displays 2fold increase in activity compared to the wild type enzyme Artemisia annua
H448S the mutant displays 3fold increase in activity compared to the wild type enzyme Artemisia annua
H448T the mutant displays 2fold increase in activity compared to the wild type enzyme Artemisia annua
H448V the mutant displays 2fold increase in activity compared to the wild type enzyme Artemisia annua
K449M the mutant displays 3-4fold increase in activity compared to the wild type enzyme Artemisia annua
L515A the mutant accumulates more alpha-bisabolol compared to the wild type enzyme Artemisia annua
L515C the mutant accumulates more alpha-bisabolol compared to the wild type enzyme Artemisia annua
L515G the mutant accumulates more alpha-bisabolol compared to the wild type enzyme Artemisia annua
L515S the mutant accumulates more alpha-bisabolol compared to the wild type enzyme Artemisia annua
L515T the mutant accumulates more alpha-bisabolol compared to the wild type enzyme Artemisia annua
Q518A the mutant displays 3-4fold increase in activity compared to the wild type enzyme Artemisia annua
Q518L the mutant displays 3-4fold increase in activity compared to the wild type enzyme Artemisia annua
T296A the mutant retains its cyclization ability producing amorpha-4,11-diene Artemisia annua
T296L the mutant loses its cyclization ability producing the acyclic beta-farnesene instead of amorpha-4,11-diene Artemisia annua
T296S the mutant retains its cyclization ability producing amorpha-4,11-diene Artemisia annua
T296V the mutant loses its cyclization ability producing the acyclic beta-farnesene instead of amorpha-4,11-diene Artemisia annua
T296Y the mutant loses its cyclization ability producing the acyclic beta-farnesene instead of amorpha-4,11-diene Artemisia annua
T399A the mutant exhibits an increase in the level of amorpha-4,7-diene Artemisia annua
T399E inactive Artemisia annua
T399F inactive Artemisia annua
T399G the mutant exhibits an increase in the level of amorpha-4,7-diene Artemisia annua
T399I the mutant exhibits an increase in the level of amorpha-4,7-diene Artemisia annua
T399K inactive Artemisia annua
T399M inactive Artemisia annua
T399N the mutant exhibits an increase in the level of amorpha-4,7-diene Artemisia annua
T399P inactive Artemisia annua
T399Q inactive Artemisia annua
T399R inactive Artemisia annua
T399S/H448A the mutations improve the kcat value by 5times but at the expense of a raised Km making its catalytic efficiency about 3 times higher compared to the wild type enzyme Artemisia annua
T399V the mutant exhibits an increase in the level of amorpha-4,7-diene Artemisia annua
V396A the mutant displays 3-4fold increase in activity compared to the wild type enzyme Artemisia annua
V396I the mutant displays 3-4fold increase in activity compared to the wild type enzyme Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Artemisia annua
-
amorpha-4,11-diene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Artemisia annua Q9AR04
-
-

Purification (Commentary)

Purification (Comment) Organism
His MultiTrap column chromatography Artemisia annua

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
Artemisia annua amorpha-4,11-diene + diphosphate
-
?

Synonyms

Synonyms Comment Organism
ADS
-
Artemisia annua