Literature summary for 4.2.3.24 extracted from

Anthony, J.R.; Anthony, L.C.; Nowroozi, F.; Kwon, G.; Newman, J.D.; Keasling, J.D.
Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene (2008), Metab. Eng., 11, 13-19.

Application

Application	Comment	Organism
drug development	amorpha-4,11-diene is an anti-malarial drug precursor	Artemisia annua

Cloned(Commentary)

Cloned (Comment)	Organism
Escherichia coli DH10B is used for cloning and strain DH1 is used for expression, developing and optimizing of a simple cloning system for expression of the amorphadiene biosynthetic pathway. The pathway genes are originally constructed by assembly of the operons onto three different plasmids, each with a different copy number and Escherichia coli promoter. Escherichia coli DH1 harboring pAM45MevT and pADS exhibits a significant increase in amorphadiene production (21 mg/l at 75 h), which is 3fold higher than the original base-strain DH1 pMevTpMBISpADS. To increase ADS expression, transforming of DH1pAM92 with pADS is done, resulting in a combined ADS gene dosage of 30-45 copies/cell. The strain exhibits an increase in amorphadiene production over DH1pAM92, but still does not approach the peak titers obtained with DH1pAM45pADS	Artemisia annua

Cloned (Comment)

Organism

Escherichia coli DH10B is used for cloning and strain DH1 is used for expression, developing and optimizing of a simple cloning system for expression of the amorphadiene biosynthetic pathway. The pathway genes are originally constructed by assembly of the operons onto three different plasmids, each with a different copy number and Escherichia coli promoter. Escherichia coli DH1 harboring pAM45MevT and pADS exhibits a significant increase in amorphadiene production (21 mg/l at 75 h), which is 3fold higher than the original base-strain DH1 pMevTpMBISpADS. To increase ADS expression, transforming of DH1pAM92 with pADS is done, resulting in a combined ADS gene dosage of 30-45 copies/cell. The strain exhibits an increase in amorphadiene production over DH1pAM92, but still does not approach the peak titers obtained with DH1pAM45pADS

Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-trans,6-trans-farnesyl diphosphate	Artemisia annua	-	amorpha-4,11-diene + diphosphate	-	?
additional information	Artemisia annua	ADS is one of the two rate-limiting enzymes in the amorphadiene biosynthetic pathway	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Artemisia annua	-	Escherichia coli strain contains plasmid-borne copies of the mevalonate pathway from Saccharomyces cerevisiae, as well as the amorphadiene synthase(ADS) gene from Artemisia annua	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-trans,6-trans-farnesyl diphosphate	-	Artemisia annua	amorpha-4,11-diene + diphosphate	-	?
additional information	ADS is one of the two rate-limiting enzymes in the amorphadiene biosynthetic pathway	Artemisia annua	?	-	?

Synonyms

Synonyms	Comment	Organism
ADS	-	Artemisia annua
amorphadiene synthase	-	Artemisia annua