Literature summary for 4.2.3.24 extracted from

Picaud, S.; Olsson, M.E.; Brodelius, P.E.
Improved conditions for production of recombinant plant sesquiterpene synthases in Escherichia coli (2007), Protein Expr. Purif., 51, 71-79.

Cloned(Commentary)

Cloned (Comment)	Organism
into the pET28 vector for expression in Escherichia coli BL21DE3, BL21DE3 Tuner, BL21DE3 pLysS and BL21DE3 pLysS Tuner cells using different inducing agents	Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-trans,6-trans-farnesyl diphosphate	Artemisia annua	-	amorpha-4,11-diene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Artemisia annua	Q9AR04	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified by immobilized metal affinity chromatography on a column loaded with Co2+	Artemisia annua

Renatured (Commentary)

Renatured (Comment)	Organism
protein is recovered from the inclusion body fraction	Artemisia annua

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000002	-	strain BL21DE3 pLysS Tuner, 6 g/l Inducer, sorbitol, betaine	Artemisia annua
0.000002	-	strain BL21DE3 pLysS Tuner, no Inducer	Artemisia annua
0.000002	-	strain BL21DE3 pLysS, without IPTG, sorbitol, betaine	Artemisia annua
0.000003	-	strain BL21DE3 pLysS Tuner, 1 g/l Inducer, sorbitol, betaine	Artemisia annua
0.000003	-	strain BL21DE3 pLysS Tuner, 3 g/l Inducer, sorbitol, betaine	Artemisia annua
0.000003	-	strain BL21DE3 pLysS Tuner, no Inducer, sorbitol, betaine	Artemisia annua
0.000008	-	strain BL21DE3 pLysS Tuner, 6 g/l Inducer	Artemisia annua
0.000008	-	strain BL21DE3 pLysS Tuner, without IPTG	Artemisia annua
0.000008	-	strain BL21DE3 pLysS Tuner, without IPTG, sorbitol, betaine	Artemisia annua
0.000013	-	strain BL21DE3 pLysS Tuner, 1 g/l Inducer	Artemisia annua
0.000013	-	strain BL21DE3 pLysS Tuner, 3 g/l Inducer	Artemisia annua
0.00002	-	strain BL21DE3 pLysS Tuner, 0.1 mM IPTG	Artemisia annua
0.00002	-	strain BL21DE3 pLysS Tuner, 0.2 mM IPTG	Artemisia annua
0.00002	-	strain BL21DE3 pLysS Tuner, 0.5 mM IPTG	Artemisia annua
0.00002	-	strain BL21DE3 pLysS, 6 g/l Inducer	Artemisia annua
0.00002	-	strain BL21DE3 pLysS, no Inducer	Artemisia annua
0.00002	-	strain BL21DE3 pLysS, no Inducer, sorbitol, betaine	Artemisia annua
0.00002	-	strain BL21DE3 pLysS, without IPTG	Artemisia annua
0.00003	-	strain BL21DE3 pLysS, 0.1 mM IPTG	Artemisia annua
0.00003	-	strain BL21DE3 pLysS, 0.2 mM IPTG	Artemisia annua
0.00003	-	strain BL21DE3 pLysS, 0.5 mM IPTG	Artemisia annua
0.00003	-	strain BL21DE3 pLysS, 1 g/l Inducer	Artemisia annua
0.00003	-	strain BL21DE3 pLysS, 1 g/l Inducer, sorbitol, betaine	Artemisia annua
0.00003	-	strain BL21DE3 pLysS, 3 g/l Inducer	Artemisia annua
0.00003	-	strain BL21DE3 pLysS, 3 g/l Inducer, sorbitol, betaine	Artemisia annua
0.00003	-	strain BL21DE3 pLysS, 6 g/l Inducer, sorbitol, betaine	Artemisia annua
0.00003	-	strain BL21DE3 Tuner, 0.1 mM IPTG	Artemisia annua
0.00003	-	strain BL21DE3 Tuner, 0.2 mM IPTG	Artemisia annua
0.00003	-	strain BL21DE3 Tuner, 0.5 mM IPTG	Artemisia annua
0.00003	-	strain BL21DE3 Tuner, no Inducer	Artemisia annua
0.00003	-	strain BL21DE3 Tuner, without IPTG	Artemisia annua
0.00003	-	strain BL21DE3, 0.1 mM IPTG	Artemisia annua
0.00003	-	strain BL21DE3, 0.2 mM IPTG	Artemisia annua
0.00003	-	strain BL21DE3, 0.5 mM IPTG	Artemisia annua
0.00003	-	strain BL21DE3, 1 g/l Inducer	Artemisia annua
0.00003	-	strain BL21DE3, 3 g/l Inducer	Artemisia annua
0.00003	-	strain BL21DE3, 6 g/l Inducer	Artemisia annua
0.00005	-	strain BL21DE3 Tuner, 1 g/l Inducer	Artemisia annua
0.00005	-	strain BL21DE3 Tuner, 3 g/l Inducer	Artemisia annua
0.00005	-	strain BL21DE3 Tuner, 6 g/l Inducer	Artemisia annua
0.00005	-	strain BL21DE3, no Inducer	Artemisia annua
0.00005	-	strain BL21DE3, without IPTG	Artemisia annua
0.00006	-	strain BL21DE3, without IPTG, sorbitol, betaine	Artemisia annua
0.00008	-	strain BL21DE3 Tuner, without IPTG, sorbitol, betaine	Artemisia annua
0.0001	-	strain BL21DE3 Tuner, 1 g/l Inducer, sorbitol, betaine	Artemisia annua
0.00012	-	strain BL21DE3 Tuner, 3 g/l Inducer, sorbitol, betaine	Artemisia annua
0.00012	-	strain BL21DE3, 1 g/l Inducer, sorbitol, betaine	Artemisia annua
0.00012	-	strain BL21DE3, no Inducer, sorbitol, betaine	Artemisia annua
0.00013	-	strain BL21DE3 Tuner, 6 g/l Inducer, sorbitol, betaine	Artemisia annua
0.00013	-	strain BL21DE3 Tuner, no Inducer, sorbitol, betaine	Artemisia annua
0.00013	-	strain BL21DE3, 3 g/l Inducer, sorbitol, betaine	Artemisia annua
0.00015	-	strain BL21DE3, 0.2 mM IPTG, sorbitol, betaine	Artemisia annua
0.00015	-	strain BL21DE3, 6 g/l Inducer, sorbitol, betaine	Artemisia annua
0.0002	-	strain BL21DE3 pLysS Tuner, 0.1 mM IPTG, sorbitol, betaine	Artemisia annua
0.0002	-	strain BL21DE3 pLysS, 0.1 mM IPTG, sorbitol, betaine	Artemisia annua
0.0002	-	strain BL21DE3 pLysS, 0.2 mM IPTG, sorbitol, betaine	Artemisia annua
0.0002	-	strain BL21DE3 pLysS, 0.5 mM IPTG, sorbitol, betaine	Artemisia annua
0.0002	-	strain BL21DE3 Tuner, 0.1 mM IPTG, sorbitol, betaine	Artemisia annua
0.0002	-	strain BL21DE3 Tuner, 0.2 mM IPTG, sorbitol, betaine	Artemisia annua
0.0002	-	strain BL21DE3 Tuner, 0.5 mM IPTG, sorbitol, betaine	Artemisia annua
0.0002	-	strain BL21DE3, 0.1 mM IPTG, sorbitol, betaine	Artemisia annua
0.0002	-	strain BL21DE3, 0.5 mM IPTG, sorbitol, betaine	Artemisia annua
0.00025	-	strain BL21DE3 pLysS Tuner, 0.2 mM IPTG, sorbitol, betaine	Artemisia annua
0.00025	-	strain BL21DE3 pLysS Tuner, 0.5 mM IPTG, sorbitol, betaine	Artemisia annua

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-trans,6-trans-farnesyl diphosphate	-	Artemisia annua	amorpha-4,11-diene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
ADS	-	Artemisia annua
amorpha-4,11-diene synthase	-	Artemisia annua

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	activity assay	Artemisia annua

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	activity assay	Artemisia annua