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Literature summary for 4.2.3.23 extracted from

  • Nguyen, T.D.; Faraldos, J.A.; Vardakou, M.; Salmon, M.; OMaille, P.E.; Ro, D.K.
    Discovery of germacrene A synthases in Barnadesia spinosa The first committed step in sesquiterpene lactone biosynthesis in the basal member of the Asteraceae (2016), Biochem. Biophys. Res. Commun., 479, 622-627 .
    View publication on PubMed
Search for more literature for 4.2.3.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae EPY300 cells Barnadesia spinosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0078
-
 (2E,6E)-farnesyl diphosphate isoform GAS1, at pH 7.0 and 23°C Barnadesia spinosa
0.0148
-
 (2E,6E)-farnesyl diphosphate isoform GAS2, at pH 7.0 and 23°C Barnadesia spinosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Barnadesia spinosa
-
 (+)-(R)-germacrene A + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Barnadesia spinosa A0A0U1XNG2
-
-
Barnadesia spinosa A0A0U1XRI2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Barnadesia spinosa (+)-(R)-germacrene A + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 66500, calculated from amino acid sequence Barnadesia spinosa
? x * 64100, calculated from amino acid sequence Barnadesia spinosa

Synonyms

Synonyms Comment Organism
GAS1 isoform Barnadesia spinosa
GAS2 isoform Barnadesia spinosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 (2E,6E)-farnesyl diphosphate isoform GAS1, at pH 7.0 and 23°C Barnadesia spinosa
0.28
-
 (2E,6E)-farnesyl diphosphate isoform GAS2, at pH 7.0 and 23°C Barnadesia spinosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17
-
 (2E,6E)-farnesyl diphosphate isoform GAS1, at pH 7.0 and 23°C Barnadesia spinosa
19
-
 (2E,6E)-farnesyl diphosphate isoform GAS2, at pH 7.0 and 23°C Barnadesia spinosa