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Literature summary for 4.2.3.23 extracted from

  • Agger, S.A.; Lopez-Gallego, F.; Hoye, T.R.; Schmidt-Dannert, C.
    Identification of sesquiterpene synthases from Nostoc punctiforme PCC 73102 and Nostoc sp. strain PCC 7120 (2008), J. Bacteriol., 190, 6084-6096.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.23

Cloned(Commentary)

Cloned (Comment) Organism
NS1 is overexpressed at high levels in Escherichia coli. The gene encoding NS1 is flanked by an ORF encoding a putative cytochrome P450 monooxygenase Nostoc sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 2-trans,6-trans-farnesyl diphosphate in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM mercaptoethanol Nostoc sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enzyme requires Mg2+ for catalysis, Mg2+ ions bind to two metal-binding motifs that are conserved among all terpene synthases Nostoc sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate Nostoc sp.
-
 (+)-(10R)-germacrene A + diphosphate
-
 ?
2-trans,6-trans-farnesyl diphosphate Nostoc sp. PCC 712
-
 (+)-(10R)-germacrene A + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Nostoc sp.
-
 strain PCC 7120, previously Anabaena sp.
-

Purification (Commentary)

Purification (Comment) Organism
cell debris is cleared by centrifugation and the cleared protein extract is used to purify proteins by metal-affinity chromatography Nostoc sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate
-
 Nostoc sp. (+)-(10R)-germacrene A + diphosphate
-
 ?
2-trans,6-trans-farnesyl diphosphate
-
 Nostoc sp. PCC 712 (+)-(10R)-germacrene A + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
germacrene A synthase
-
 Nostoc sp.
NS1 sesquiterpene synthase Nostoc sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
 2-trans,6-trans-farnesyl diphosphate determined by incubating purified enzyme with various concentrations of 1 to 100 microM 2-trans,6-trans-farnesyl diphosphate Nostoc sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Nostoc sp.

Cofactor

Cofactor Comment Organism Structure
NADPH assay at Nostoc sp.