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Literature summary for 4.2.3.23 extracted from

  • Bouwmeester, H.J.; Kodde, J.; Verstappen, F.W.; Altug, I.G.; de Kraker, J.W.; Wallaart, T.E.
    Isolation and characterization of two germacrene A synthase cDNA clones from chicory (2002), Plant Physiol., 129, 134-144.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.23

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli Cichorium intybus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Cichorium intybus
0.0032
-
 2-trans,6-trans-farnesyl diphosphate pH 7.0, 30°C Cichorium intybus
0.0069
-
 2-trans,6-trans-farnesyl diphosphate pH 7.0, 30°C Cichorium intybus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate Cichorium intybus
-
 (+)-(10R)-germacrene A + diphosphate
-
 ?
additional information Cichorium intybus (+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cichorium intybus Q8LSC2 short enzyme form, mRNA; 2 isozymes encoded by 2 different genes
-
Cichorium intybus Q8LSC3 long enzyme form, mRNA; 2 isozymes encoded by 2 different genes
-

Purification (Commentary)

Purification (Comment) Organism
recombinant long isozyme from Escherichia coli, native isozyme partially from chicory heads by anion exchange chromatography Cichorium intybus
recombinant short isozyme from Escherichia coli, native isozyme partially from chicory heads by anion exchange chromatography Cichorium intybus

Reaction

Reaction Comment Organism Reaction ID
(2E,6E)-farnesyl diphosphate = (+)-(R)-gemacrene A + diphosphate structure modeling and reaction mechanism, overview Cichorium intybus
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf head leaves, very low expression in green leaves Cichorium intybus
-
additional information both isozymes are expressed in all tissues but in varying levels, overview Cichorium intybus
-
additional information nearly no expression of the short isozyme in head leaves and in green leaves, both isozymes are expressed in all tissues but in varying levels, overview Cichorium intybus
-
seedling normal and etiolated Cichorium intybus
-
shoot head core tissue Cichorium intybus
-
taproot especially in outer tissue, highest expression level of the long isozyme Cichorium intybus
-
taproot highest expression level of the short isozyme in outer and inner tissues Cichorium intybus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate
-
 Cichorium intybus (+)-(10R)-germacrene A + diphosphate
-
 ?
additional information (+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory Cichorium intybus ?
-
 ?

Subunits

Subunits Comment Organism
More isozyme primary sequence and three-dimensional structure modeling Cichorium intybus

Synonyms

Synonyms Comment Organism
GASlo
-
 Cichorium intybus
GASsh
-
 Cichorium intybus
germacrene A synthase
-
 Cichorium intybus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Cichorium intybus
7
-
-
 Cichorium intybus