Literature summary for 4.2.3.23 extracted from

de Kraker, J.W.; Franssen, M.C.; de Groot, A.; Konig, W.A.; Bouwmeester, H.J.
(+)-Germacrene A biosynthesis. The committed step in the biosynthesis of bitter sesquiterpene lactones in chicory (1998), Plant Physiol., 117, 1381-1392.

Search for more literature for 4.2.3.23

Activating Compound

Activating Compound	Comment	Organism	Structure
Tween 20	at 0.1%, activates the enzyme by 5fold	Cichorium intybus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0066	-	2-trans,6-trans-farnesyl diphosphate	pH 7.0, 30°C	Cichorium intybus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Cichorium intybus	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Cichorium intybus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	gel filtration	Cichorium intybus
56000	-	1 * 56000, about, SDS-PAGE	Cichorium intybus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-trans,6-trans-farnesyl diphosphate	Cichorium intybus	the product germacerene A is released after reaction to be further processing by oxidations and/or glucosylations and cyclization by a germacrane cyclase	(+)-(10R)-germacrene A + diphosphate	-	?
additional information	Cichorium intybus	(+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Cichorium intybus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
201fold from roots by anion exchange and dye-ligand affinity chromatography	Cichorium intybus

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Cichorium intybus	-
root	high enzyme activity	Cichorium intybus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.24	-	purified enzyme	Cichorium intybus

Storage Stability

Storage Stability	Organism
-80°C, partially purified root enzyme, in 15 mM MOPS, pH 7.0, 10 mM MgCl2, 2 mM DTT, 30% v/v glycerol, stable for several months	Cichorium intybus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-trans,6-trans-farnesyl diphosphate	-	Cichorium intybus	(+)-(10R)-germacrene A + diphosphate	reaction product solubilization and analysis in pentane, determination of germacrene A configuration	?
2-trans,6-trans-farnesyl diphosphate	the product germacerene A is released after reaction to be further processing by oxidations and/or glucosylations and cyclization by a germacrane cyclase	Cichorium intybus	(+)-(10R)-germacrene A + diphosphate	-	?
additional information	(+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory	Cichorium intybus	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 56000, about, SDS-PAGE	Cichorium intybus

Synonyms

Synonyms	Comment	Organism
(+)-germacrene A synthase	-	Cichorium intybus
sesquiterpene cyclase	-	Cichorium intybus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Cichorium intybus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.7	-	about, broad optimum	Cichorium intybus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.1	7.3	half maximal activities at pH 5.1 and pH 7.3	Cichorium intybus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)