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Literature summary for 4.2.3.23 extracted from
- (+)-(10R)-Germacrene A synthase from goldenrod, Solidago canadensis; cDNA isolation, bacterial expression and functional analysis (2002), Phytochemistry, 60, 691-702.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA library construction, phage display, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli as N-terminal thioredoxin fusion protein | Solidago canadensis |
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes the enzyme | Solidago canadensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant enzyme | Solidago canadensis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Solidago canadensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64700 | - |
x * 64700, about, DNA sequence calculation | Solidago canadensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-trans,6-trans-farnesyl diphosphate | Solidago canadensis | - |
(+)-(10R)-germacrene A + diphosphate | 98% of total product is (+)-(10R)-germacrene A, 2% is alpha-humulene in feeding experiments in vivo | ? | |
| additional information | Solidago canadensis | sesquiterpene hydrocarbon profile of leaf extracts, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solidago canadensis | Q9AR67 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant thioredoxin fusion enzyme from Escherichia coli by nickel chelate affinity chromatography | Solidago canadensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Solidago canadensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-trans,6-trans-farnesyl diphosphate | - |
Solidago canadensis | (+)-(10R)-germacrene A + diphosphate | 98% of total product is (+)-(10R)-germacrene A, 2% is alpha-humulene in feeding experiments in vivo | ? | |
| 2-trans,6-trans-farnesyl diphosphate | - |
Solidago canadensis | (+)-(10R)-germacrene A + diphosphate | chiral GC-MS product analysis | ? | |
| additional information | sesquiterpene hydrocarbon profile of leaf extracts, overview | Solidago canadensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64700, about, DNA sequence calculation | Solidago canadensis |
| More | molecular modeling, structure analysis, enzyme contains the conserved motif DDXXD/E | Solidago canadensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (+)-(10R)-germacrene A synthase | - |
Solidago canadensis |
| Sc1 | - |
Solidago canadensis |
| sesquiterpene synthase | - |
Solidago canadensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Solidago canadensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Solidago canadensis |
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Release 2023.1 (January 2023)
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