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Literature summary for 4.2.3.23 extracted from

  • Chang, Y.J.; Jin, J.; Nam, H.Y.; Kim, S.U.
    Point mutation of (+)-germacrene A synthase from Ixeris dentata (2005), Biotechnol. Lett., 27, 285-288.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in bacteria Ixeridium dentatum

Protein Variants

Protein Variants Comment Organism
A435T site-directed mutagenesis, altered kinetics but similar activity compared to the wild-type enzyme Ixeridium dentatum
S434A site-directed mutagenesis, activity and kinetics similar to the wild-type enzyme Ixeridium dentatum
S434T/A435T site-directed mutagenesis, inactive mutant Ixeridium dentatum
T433A site-directed mutagenesis, altered kinetics and reduced activity compared to the wild-type enzyme Ixeridium dentatum
T433A/A435T site-directed mutagenesis, altered kinetics and reduced activity compared to the wild-type enzyme Ixeridium dentatum
T433A/S434A site-directed mutagenesis, inactive mutant Ixeridium dentatum
T433A/S434A/A435T site-directed mutagenesis, inactive mutant Ixeridium dentatum
T433A/S434T site-directed mutagenesis, inactive mutant Ixeridium dentatum

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Ixeridium dentatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0047
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant mutant T433A Ixeridium dentatum
0.0076
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant mutant T433A/A435T Ixeridium dentatum
0.0106
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant mutant S434A Ixeridium dentatum
0.011
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant wild-type enzyme Ixeridium dentatum
0.0251
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant mutant A435T Ixeridium dentatum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Ixeridium dentatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate Ixeridium dentatum
-
(+)-(10R)-germacrene A + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Ixeridium dentatum Q6YN71
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from bacteria Ixeridium dentatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate
-
Ixeridium dentatum (+)-(10R)-germacrene A + diphosphate
-
?
2-trans,6-trans-farnesyl diphosphate
-
Ixeridium dentatum (+)-(10R)-germacrene A + diphosphate GC-MS product analysis ?

Synonyms

Synonyms Comment Organism
(+)-germacrene A synthase
-
Ixeridium dentatum
GAS
-
Ixeridium dentatum
guaiadiene synthase
-
Ixeridium dentatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Ixeridium dentatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00035
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant mutant T433A Ixeridium dentatum
0.00037
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant mutant T433A/A435T Ixeridium dentatum
0.016
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant wild-type enzyme Ixeridium dentatum
0.017
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant mutant S434A Ixeridium dentatum
0.044
-
2-trans,6-trans-farnesyl diphosphate pH 7.0, 25°C, recombinant mutant A435T Ixeridium dentatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Ixeridium dentatum