Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in bacteria | Ixeridium dentatum |
Protein Variants | Comment | Organism |
---|---|---|
A435T | site-directed mutagenesis, altered kinetics but similar activity compared to the wild-type enzyme | Ixeridium dentatum |
S434A | site-directed mutagenesis, activity and kinetics similar to the wild-type enzyme | Ixeridium dentatum |
S434T/A435T | site-directed mutagenesis, inactive mutant | Ixeridium dentatum |
T433A | site-directed mutagenesis, altered kinetics and reduced activity compared to the wild-type enzyme | Ixeridium dentatum |
T433A/A435T | site-directed mutagenesis, altered kinetics and reduced activity compared to the wild-type enzyme | Ixeridium dentatum |
T433A/S434A | site-directed mutagenesis, inactive mutant | Ixeridium dentatum |
T433A/S434A/A435T | site-directed mutagenesis, inactive mutant | Ixeridium dentatum |
T433A/S434T | site-directed mutagenesis, inactive mutant | Ixeridium dentatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Ixeridium dentatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant mutant T433A | Ixeridium dentatum | |
0.0076 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant mutant T433A/A435T | Ixeridium dentatum | |
0.0106 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant mutant S434A | Ixeridium dentatum | |
0.011 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant wild-type enzyme | Ixeridium dentatum | |
0.0251 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant mutant A435T | Ixeridium dentatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Ixeridium dentatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | Ixeridium dentatum | - |
(+)-(10R)-germacrene A + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ixeridium dentatum | Q6YN71 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from bacteria | Ixeridium dentatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | - |
Ixeridium dentatum | (+)-(10R)-germacrene A + diphosphate | - |
? | |
2-trans,6-trans-farnesyl diphosphate | - |
Ixeridium dentatum | (+)-(10R)-germacrene A + diphosphate | GC-MS product analysis | ? |
Synonyms | Comment | Organism |
---|---|---|
(+)-germacrene A synthase | - |
Ixeridium dentatum |
GAS | - |
Ixeridium dentatum |
guaiadiene synthase | - |
Ixeridium dentatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Ixeridium dentatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant mutant T433A | Ixeridium dentatum | |
0.00037 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant mutant T433A/A435T | Ixeridium dentatum | |
0.016 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant wild-type enzyme | Ixeridium dentatum | |
0.017 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant mutant S434A | Ixeridium dentatum | |
0.044 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant mutant A435T | Ixeridium dentatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Ixeridium dentatum |