Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.23 extracted from

  • Bertea, C.M.; Voster, A.; Verstappen, F.W.; Maffei, M.; Beekwilder, J.; Bouwmeester, H.J.
    Isoprenoid biosynthesis in Artemisia annua: cloning and heterologous expression of a germacrene A synthase from a glandular trichome cDNA library (2006), Arch. Biochem. Biophys., 448, 3-12.
    View publication on PubMed
Search for more literature for 4.2.3.23

Cloned(Commentary)

Cloned (Comment) Organism
cloning from a constructed glandular trichome cDNA library, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Escherichia coli strain BL21-DE3-RIL Artemisia annua

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate Artemisia annua
-
 (+)-(10R)-germacrene A + diphosphate
-
 ?
additional information Artemisia annua (+)-germacrene A synthesis is a step in isoprenoid biosynthesis, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Artemisia annua Q1PDD2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Artemisia annua
-
trichome
-
 Artemisia annua
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate
-
 Artemisia annua (+)-(10R)-germacrene A + diphosphate
-
 ?
2-trans,6-trans-farnesyl diphosphate
-
 Artemisia annua (+)-(10R)-germacrene A + diphosphate GC-MS product analysis ?
additional information (+)-germacrene A synthesis is a step in isoprenoid biosynthesis, overview Artemisia annua ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Artemisia annua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Artemisia annua