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Literature summary for 4.2.3.203 extracted from

  • Ringel, M.; Reinbold, M.; Hirte, M.; Haack, M.; Huber, C.; Eisenreich, W.; Masri, M.; Schenk, G.; Guddat, L.; Loll, B.; Kerr, R.; Garbe, D.; Brueck, T.
    Towards a sustainable generation of pseudopterosin-type bioactives (2020), Green Chem., 22, 6033-6046.
No PubMed abstract available
Search for more literature for 4.2.3.203

Application

Application Comment Organism
synthesis expression in Escherichia coli host gives a total terpene yield of mutant M75L of 41.91 mg/. Lipase catalysed chemo-enzymatic oxidation differentially transforms the isomers isoelisabethatriene A and B to the advanced pseudopterosin precursor erogorgiaene (yield: 69%) and the compound 1R-epoxy-elisabetha-5,14-diene (yield: 41%), respectively Streptomyces clavuligerus

Protein Variants

Protein Variants Comment Organism
A79F inactive Streptomyces clavuligerus
G182A mutant displays an altered product spectrum Streptomyces clavuligerus
G182F mutant displays an altered product spectrum Streptomyces clavuligerus
G182K mutation does not affect the product spectrum Streptomyces clavuligerus
H184A mutant displays an altered product spectrum Streptomyces clavuligerus
H184F mutant displays an altered product spectrum Streptomyces clavuligerus
L54A inactive Streptomyces clavuligerus
M188A inactive Streptomyces clavuligerus
M188G inactive Streptomyces clavuligerus
M188K inactive Streptomyces clavuligerus
M188Y inactive Streptomyces clavuligerus
M300G inactive Streptomyces clavuligerus
M300I mutant displays an altered product spectrum with decrease in isoelisabethatriene A production and a concomitant increase in isoelisabethatriene B Streptomyces clavuligerus
M304C mutant displays an altered product spectrum with decrease in isoelisabethatriene A production and a concomitant increase in isoelisabethatriene B Streptomyces clavuligerus
M304D inactive Streptomyces clavuligerus
M71G inactive Streptomyces clavuligerus
M71P inactive Streptomyces clavuligerus
M71R inactive Streptomyces clavuligerus
M71Y mutant displays a significantly enhanced yield of isoelisabethatriene A and B, with a concomitant reduction in hydroperene and hydropyrol production Streptomyces clavuligerus
M75F mutant displays a significantly enhanced yield of isoelisabethatriene A and B, with a concomitant reduction in hydroperene and hydropyrol production. M75F shows the highest isoelisabethatriene B yield observed Streptomyces clavuligerus
M75L mutant displays a significantly enhanced yield of isoelisabethatriene A and B, with a concomitant reduction in hydroperene and hydropyrol production. M75L shows the highest isoelisabethatriene A yield observed Streptomyces clavuligerus
R179A inactive Streptomyces clavuligerus
R313A inactive Streptomyces clavuligerus
W307A inactive Streptomyces clavuligerus
W307F mutation does not affect the product spectrum Streptomyces clavuligerus
W307G inactive Streptomyces clavuligerus
Y153A mutation does not affect the product spectrum Streptomyces clavuligerus
Y153F mutation does not affect the product spectrum Streptomyces clavuligerus
Y153R inactive Streptomyces clavuligerus
Y58A inactive Streptomyces clavuligerus
Y78A inactive Streptomyces clavuligerus

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
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 Streptomyces clavuligerus hydropyrene + hydropyrenol + isoelisabethatriene A + isoelisabethatriene B + diphosphate
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 ?

Synonyms

Synonyms Comment Organism
TPS5
-
 Streptomyces clavuligerus