Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells | Citrus sinensis |
Crystallization (Comment) | Organism |
---|---|
in complex with 2-fluorogeranyl diphosphate or 2-fluoroneryl diphosphate, vapor diffusion method | Citrus sinensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Fluorogeranyl diphosphate | competitive inhibitor | Citrus sinensis | |
2-fluoroneryl diphosphate | competitive inhibitor | Citrus sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | Citrus sinensis | - |
(R)-limonene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus sinensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity column chromatography | Citrus sinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-fluorogeranyl diphosphate | - |
Citrus sinensis | ? | - |
? | |
2-fluoroneryl diphosphate | - |
Citrus sinensis | ? | - |
? | |
geranyl diphosphate | - |
Citrus sinensis | (R)-limonene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(+)-limonene synthase | - |
Citrus sinensis |
(+)-LS | - |
Citrus sinensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00024 | - |
2-fluoroneryl diphosphate | pH and temperature not specified in the publication | Citrus sinensis | |
0.00054 | - |
2-Fluorogeranyl diphosphate | pH and temperature not specified in the publication | Citrus sinensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
2-Fluorogeranyl diphosphate | pH and temperature not specified in the publication | Citrus sinensis | |
0.0395 | - |
2-fluoroneryl diphosphate | pH and temperature not specified in the publication | Citrus sinensis |