Literature summary for 4.2.3.20 extracted from

Morehouse, B.R.; Kumar, R.P.; Matos, J.O.; Olsen, S.N.; Entova, S.; Oprian, D.D.
Functional and structural characterization of a (+)-limonene synthase from Citrus sinensis (2017), Biochemistry, 56, 1706-1715 .

Search for more literature for 4.2.3.20

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Citrus sinensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoenzyme, sitting drop vapor diffusion method, using 12-16% (w/v) PEG 8000 as precipitant	Citrus sinensis

Inhibitors

Inhibitors	Comment	Organism	Structure
Mn2+	the enzyme is strongly inhibited at concentrations of more than 1 mM	Citrus sinensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0131	-	geranyl diphosphate	at pH 8.0, temperature not specified in the publication	Citrus sinensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	may replace Mn2+ but with much lower efficiency (maximum activity at 20 mM)	Citrus sinensis
Mn2+	required for activity, 0.4 mM used in assay conditions	Citrus sinensis
additional information	not affected by K+ and Na+	Citrus sinensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	gel filtration	Citrus sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranyl diphosphate	Citrus sinensis	-	(R)-limonene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Citrus sinensis	A0A1C9J6A7	navel orange	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-Sepharose column chromatography	Citrus sinensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate	-	Citrus sinensis	(R)-limonene + diphosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 70000, SDS-PAGE	Citrus sinensis
monomer	1 * 65700, calculated from amino acid sequence	Citrus sinensis

Synonyms

Synonyms	Comment	Organism
(+)-limonene synthase	-	Citrus sinensis
(+)-LS	-	Citrus sinensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.186	-	geranyl diphosphate	at pH 8.0, temperature not specified in the publication	Citrus sinensis

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Release 2023.1 (January 2023)