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show all sequences of 4.2.3.2

Kinetic characterization of the human O-phosphoethanolamine phospho-lyase reveals unconventional features of this specialized pyridoxal phosphate-dependent lyase

Schiroli, D.; Ronda, L.; Peracchi, A.; FEBS J. 282, 183-199 (2015)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-Aminopropylphosphonate
-
Homo sapiens
ethylsulfonate
-
Homo sapiens
methylsulfonate
-
Homo sapiens
phosphate
competitive inhibitor at pH values between 6.0 and 8.0
Homo sapiens
sulfate
competitive inhibitor at pH values between 6.0 and 8.0
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
Ethanolamine phosphate
at pH 7.4 and 30°C
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ethanolamine phosphate + H2O
Homo sapiens
-
acetaldehyde + NH3 + phosphate
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanolamine phosphate + H2O
-
747739
Homo sapiens
acetaldehyde + NH3 + phosphate
-
-
-
ir
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.9
-
Ethanolamine phosphate
at pH 6.0 and 30°C
Homo sapiens
1.2
-
Ethanolamine phosphate
at pH 7.4 and 30°C
Homo sapiens
2.3
-
Ethanolamine phosphate
at pH 8.0 and 30°C
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.5
-
phosphate
at pH 8.0 and 30°C
Homo sapiens
1.5
-
sulfate
at pH 6.0 and 30°C; at pH 8.0 and 30°C
Homo sapiens
8
-
phosphate
at pH 6.0 and 30°C
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-Aminopropylphosphonate
-
Homo sapiens
ethylsulfonate
-
Homo sapiens
methylsulfonate
-
Homo sapiens
phosphate
competitive inhibitor at pH values between 6.0 and 8.0
Homo sapiens
sulfate
competitive inhibitor at pH values between 6.0 and 8.0
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.5
-
phosphate
at pH 8.0 and 30°C
Homo sapiens
1.5
-
sulfate
at pH 6.0 and 30°C; at pH 8.0 and 30°C
Homo sapiens
8
-
phosphate
at pH 6.0 and 30°C
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
Ethanolamine phosphate
at pH 7.4 and 30°C
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ethanolamine phosphate + H2O
Homo sapiens
-
acetaldehyde + NH3 + phosphate
-
-
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanolamine phosphate + H2O
-
747739
Homo sapiens
acetaldehyde + NH3 + phosphate
-
-
-
ir
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.9
-
Ethanolamine phosphate
at pH 6.0 and 30°C
Homo sapiens
1.2
-
Ethanolamine phosphate
at pH 7.4 and 30°C
Homo sapiens
2.3
-
Ethanolamine phosphate
at pH 8.0 and 30°C
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1
-
Ethanolamine phosphate
at pH 7.4 and 30°C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1
-
Ethanolamine phosphate
at pH 7.4 and 30°C
Homo sapiens
Other publictions for EC 4.2.3.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747739
Schiroli
Kinetic characterization of t ...
Homo sapiens
FEBS J.
282
183-199
2015
-
-
-
-
-
-
5
1
-
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
3
-
-
-
1
3
-
-
-
-
-
1
-
-
-
-
5
3
1
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
1
1
137599
Faulkner
-
Phosphorylation of ethanolamin ...
Achromobacter sp., Flavobacterium rhenanum, Microbacterium arborescens
Biochem. Soc. Trans.
2
133-136
1974
-
-
-
-
-
-
3
3
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137598
Jones
Microbial metabolism of amino ...
Pantoea agglomerans, Pantoea ananatis, Pectobacterium carotovorum
Biochem. J.
134
959-968
1973
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
3
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137597
Fleshood
The metabolism of O-phosphoryl ...
Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
245
4414-4420
1970
-
-
-
-
-
-
19
2
-
-
1
-
-
2
-
-
2
-
-
2
2
-
2
-
-
-
-
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
19
-
2
-
-
1
-
-
-
-
2
-
2
2
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-