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show all sequences of 4.2.3.2

The metabolism of O-phosphorylethanolamine in animal tissues. I. O-phosphorylethanolamine phospho-lyase: partial purification and characterization

Fleshood, H.L.; Pitot, H.C.; J. Biol. Chem. 245, 4414-4420 (1970)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
0.1 mM, 92% inhibition
Oryctolagus cuniculus
Ag+
0.1 mM, 92% inhibition
Rattus norvegicus
Cd2+
0.1 mM, 100% inhibition
Oryctolagus cuniculus
Cd2+
0.1 mM, 100% inhibition
Rattus norvegicus
CN-
1 mM, 88% inhibition
Oryctolagus cuniculus
CN-
1 mM, 88% inhibition
Rattus norvegicus
Co2+
0.1 mM, 25% inhibition
Oryctolagus cuniculus
Co2+
0.1 mM, 25% inhibition
Rattus norvegicus
Cu2+
0.1 mM, 94% inhibition
Oryctolagus cuniculus
Cu2+
0.1 mM, 94% inhibition
Rattus norvegicus
NaBH4
-
Oryctolagus cuniculus
p-chloromercuribenzoate
0.1 mM, 98.4% inhibition
Oryctolagus cuniculus
p-chloromercuribenzoate
0.1 mM, 98.4% inhibition
Rattus norvegicus
Pb2+
0.1 mM, 75% inhibition
Oryctolagus cuniculus
Pb2+
0.1 mM, 75% inhibition
Rattus norvegicus
SO42-
10 mM, 49% inhibition
Oryctolagus cuniculus
SO42-
10 mM, 49% inhibition
Rattus norvegicus
Zn2+
0.1 mM, 91% inhibition
Oryctolagus cuniculus
Zn2+
0.1 mM, 91% inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.61
-
Ethanolamine phosphate
-
Rattus norvegicus
0.63
-
Ethanolamine phosphate
-
Oryctolagus cuniculus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
168000
-
gel filtration
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Oryctolagus cuniculus
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.073
-
-
Rattus norvegicus
0.914
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanolamine phosphate + H2O
-
137597
Rattus norvegicus
acetaldehyde + NH3 + phosphate
-
137597
Rattus norvegicus
?
ethanolamine phosphate + H2O
-
137597
Oryctolagus cuniculus
acetaldehyde + NH3 + phosphate
-
137597
Oryctolagus cuniculus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Oryctolagus cuniculus
7.5
8
-
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.7
8.6
less than 50% of maximal activity above and below
Oryctolagus cuniculus
6.7
8.6
less than 50% of maximal activity above and below
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required, Km: 0.00027 mM
Oryctolagus cuniculus
pyridoxal 5'-phosphate
required, Km: 0.00027 mM
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required, Km: 0.00027 mM
Oryctolagus cuniculus
pyridoxal 5'-phosphate
required, Km: 0.00027 mM
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
0.1 mM, 92% inhibition
Oryctolagus cuniculus
Ag+
0.1 mM, 92% inhibition
Rattus norvegicus
Cd2+
0.1 mM, 100% inhibition
Oryctolagus cuniculus
Cd2+
0.1 mM, 100% inhibition
Rattus norvegicus
CN-
1 mM, 88% inhibition
Oryctolagus cuniculus
CN-
1 mM, 88% inhibition
Rattus norvegicus
Co2+
0.1 mM, 25% inhibition
Oryctolagus cuniculus
Co2+
0.1 mM, 25% inhibition
Rattus norvegicus
Cu2+
0.1 mM, 94% inhibition
Oryctolagus cuniculus
Cu2+
0.1 mM, 94% inhibition
Rattus norvegicus
NaBH4
-
Oryctolagus cuniculus
p-chloromercuribenzoate
0.1 mM, 98.4% inhibition
Oryctolagus cuniculus
p-chloromercuribenzoate
0.1 mM, 98.4% inhibition
Rattus norvegicus
Pb2+
0.1 mM, 75% inhibition
Oryctolagus cuniculus
Pb2+
0.1 mM, 75% inhibition
Rattus norvegicus
SO42-
10 mM, 49% inhibition
Oryctolagus cuniculus
SO42-
10 mM, 49% inhibition
Rattus norvegicus
Zn2+
0.1 mM, 91% inhibition
Oryctolagus cuniculus
Zn2+
0.1 mM, 91% inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.61
-
Ethanolamine phosphate
-
Rattus norvegicus
0.63
-
Ethanolamine phosphate
-
Oryctolagus cuniculus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
168000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Oryctolagus cuniculus
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.073
-
-
Rattus norvegicus
0.914
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanolamine phosphate + H2O
-
137597
Rattus norvegicus
acetaldehyde + NH3 + phosphate
-
137597
Rattus norvegicus
?
ethanolamine phosphate + H2O
-
137597
Oryctolagus cuniculus
acetaldehyde + NH3 + phosphate
-
137597
Oryctolagus cuniculus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Oryctolagus cuniculus
7.5
8
-
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.7
8.6
less than 50% of maximal activity above and below
Oryctolagus cuniculus
6.7
8.6
less than 50% of maximal activity above and below
Rattus norvegicus
Other publictions for EC 4.2.3.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747739
Schiroli
Kinetic characterization of t ...
Homo sapiens
FEBS J.
282
183-199
2015
-
-
-
-
-
-
5
1
-
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
3
-
-
-
1
3
-
-
-
-
-
1
-
-
-
-
5
3
1
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
1
1
137599
Faulkner
-
Phosphorylation of ethanolamin ...
Achromobacter sp., Flavobacterium rhenanum, Microbacterium arborescens
Biochem. Soc. Trans.
2
133-136
1974
-
-
-
-
-
-
3
3
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137598
Jones
Microbial metabolism of amino ...
Pantoea agglomerans, Pantoea ananatis, Pectobacterium carotovorum
Biochem. J.
134
959-968
1973
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
3
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137597
Fleshood
The metabolism of O-phosphoryl ...
Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
245
4414-4420
1970
-
-
-
-
-
-
19
2
-
-
1
-
-
2
-
-
2
-
-
2
2
-
2
-
-
-
-
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
19
-
2
-
-
1
-
-
-
-
2
-
2
2
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-