Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta (DE3) | Salvia miltiorrhiza |
transient expression in Nicotiana benthamiana | Salvia miltiorrhiza |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01044 | - |
(2E,6E)-farnesyl-diphosphate | 30°C, pH 7.0, enzyme form SmSTPS3 | Salvia miltiorrhiza | |
0.0242 | - |
(2E,6E)-farnesyl-diphosphate | 30°C, pH 7.0, enzyme form SmSTPS2 | Salvia miltiorrhiza | |
0.02535 | - |
(2E,6E)-farnesyl-diphosphate | 30°C, pH 7.0, enzyme form SmSTPS1 | Salvia miltiorrhiza |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | metal cofactor | Salvia miltiorrhiza |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl-diphosphate | Salvia miltiorrhiza | - |
(-)-5-epieremophilene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia miltiorrhiza | A0A1W6GW06 | - |
- |
Salvia miltiorrhiza | A0A1W6GW18 | - |
- |
Salvia miltiorrhiza | A0A1W6GW32 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Salvia miltiorrhiza |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
inflorescence | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root | Salvia miltiorrhiza | - |
inflorescence | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed | Salvia miltiorrhiza | - |
inflorescence | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS3 is expressed predominantly in inflorescence | Salvia miltiorrhiza | - |
leaf | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root | Salvia miltiorrhiza | - |
leaf | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS1 is expressed at a higher level than the SmSTPS2 and SmSTPS3, and its transcripts are most abundant in leaf | Salvia miltiorrhiza | - |
leaf | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed and exhibits a clear expression only in leaf | Salvia miltiorrhiza | - |
root | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root | Salvia miltiorrhiza | - |
root | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed | Salvia miltiorrhiza | - |
stem | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root | Salvia miltiorrhiza | - |
stem | mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed | Salvia miltiorrhiza | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl-diphosphate | - |
Salvia miltiorrhiza | (-)-5-epieremophilene + diphosphate | - |
? | |
(2E,6E)-farnesyl-diphosphate | (-)-5-epieremophilene i.e. (3R,4aS,5S)-4a,5-dimethyl-3-(prop-1-en-2-yl)-1,2,3,4,4a,5,6,7-octahydronaphthalene. Salvia miltiorrhiza produces three different forms of the enzyme, encoded by paralogous genes, that exhibit different spacial expression patterns and respond differently to hormone treatment (STPS1, STP2m and STP3) | Salvia miltiorrhiza | (-)-5-epieremophilene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
STP2 | - |
Salvia miltiorrhiza |
STP3 | - |
Salvia miltiorrhiza |
STPS1 | - |
Salvia miltiorrhiza |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Salvia miltiorrhiza |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.53 | - |
(2E,6E)-farnesyl-diphosphate | 30°C, pH 7.0, enzyme form SmSTPS3 | Salvia miltiorrhiza | |
2.07 | - |
(2E,6E)-farnesyl-diphosphate | 30°C, pH 7.0, enzyme form SmSTPS2 | Salvia miltiorrhiza | |
2.09 | - |
(2E,6E)-farnesyl-diphosphate | 30°C, pH 7.0, enzyme form SmSTPS1 | Salvia miltiorrhiza |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Salvia miltiorrhiza |
Organism | Comment | Expression |
---|---|---|
Salvia miltiorrhiza | SmSTPS1 is induced strongly by ethylene and moderately by methyl jasmonate, abscicic acid and gibberellin A3 | up |
Salvia miltiorrhiza | SmSTPS2 is induced by ethylene or by abscicic acid | up |
Salvia miltiorrhiza | SmSTPS3 is up-regulated slightly only by abscicic acid | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
82.7 | - |
(2E,6E)-farnesyl-diphosphate | 30°C, pH 7.0, enzyme form SmSTPS1 | Salvia miltiorrhiza | |
85.6 | - |
(2E,6E)-farnesyl-diphosphate | 30°C, pH 7.0, enzyme form SmSTPS2 | Salvia miltiorrhiza | |
148 | - |
(2E,6E)-farnesyl-diphosphate | 30°C, pH 7.0, enzyme form SmSTPS3 | Salvia miltiorrhiza |