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Literature summary for 4.2.3.195 extracted from

  • Vaughan, M.M.; Wang, Q.; Webster, F.X.; Kiemle, D.; Hong, Y.J.; Tantillo, D.J.; Coates, R.M.; Wray, A.T.; Askew, W.; ODonnell, C.; Tokuhisa, J.G.; Tholl, D.
    Formation of the unusual semivolatile diterpene rhizathalene by the Arabidopsis class I terpene synthase TPS08 in the root stele is involved in defense against belowground herbivory (2013), Plant Cell, 25, 1108-1125 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 geranylgeranyl diphosphate at 30°C, pH not specified in the publication Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Arabidopsis thaliana
-
 rhizathalene A + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O65435
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-
stele
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
 Arabidopsis thaliana rhizathalene A + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
TPS08
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction Tps08 knockout mutants are more susceptible to Bradysia larval feeding Arabidopsis thaliana
physiological function the enzyme is involved in defense against belowground herbivory like the opportunistic root herbivore fungus gnat Bradysia spp. Arabidopsis thaliana