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Literature summary for 4.2.3.19 extracted from

  • Roy, A.; Roberts, F.G.; Wilderman, P.R.; Zhou, K.; Peters, R.J.; Coates, R.M.
    16-Aza-ent-beyerane and 16-Aza-ent-trachylobane: potent mechanism-based inhibitors of recombinant ent-kaurene synthase from Arabidopsis thaliana (2007), J. Am. Chem. Soc., 129, 12453-12460.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.19

Cloned(Commentary)

Cloned (Comment) Organism
as a GST-fusion protein for expression in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
16-aza-ent-beyerane
-
 Arabidopsis thaliana
16-aza-ent-trachylobane
-
 Arabidopsis thaliana
ent-beyeran-16-yl diphosphate
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 [15-3H]ent-copalyl diphosphate pseudo-binding constant for [15-3H]ent-copalyl diphosphate with GST-recombinant kaurene synthase Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Arabidopsis thaliana
-
 ent-kaurene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SAK2 chloroplast precursor
-

Purification (Commentary)

Purification (Comment) Organism
purified by affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
 Arabidopsis thaliana ent-kaurene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ent-kaurene synthase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 activity assay at room temperature Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 activity assay Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000004
-
 in the presence of diphosphate Arabidopsis thaliana 16-aza-ent-beyerane
0.00002
-
 in the presence of diphosphate Arabidopsis thaliana 16-aza-ent-trachylobane
0.0001
-
 in the absence of diphosphate Arabidopsis thaliana 16-aza-ent-beyerane
0.001
-
 in the absence of diphosphate Arabidopsis thaliana 16-aza-ent-trachylobane
0.01
-
 in the absence of diphosphate Arabidopsis thaliana ent-beyeran-16-yl diphosphate