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Literature summary for 4.2.3.19 extracted from

  • Shen-Miller, J.; West, C.A.
    Kaurene synthetase activity in Helianthus annuus. Increases in enzyme activity after storage of seedlings in liquid nitrogen (1984), Plant Physiol., 74, 439-441.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
no activity in fresh crude extracts from seedlings for ent-kaurene formation from geranyl-geranyl diphosphate, activity detected after some days of storage in liquid nitrogen, little effect of liquid nitrogen storage on EC 4.2.3.19 activity Helianthus annuus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Helianthus annuus involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
?
ent-copalyl diphosphate Marah macrocarpa involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Helianthus annuus
-
-
-
Marah macrocarpa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling no activity in fresh crude extracts from seedlings for ent-kaurene formation from geranyl-geranyl diphosphate, activity detected after some days of storage in liquid nitrogen, little effect of liquid nitrogen storage on EC 4.2.3.19 activity Helianthus annuus
-

Storage Stability

Storage Stability Organism
-180°C, no activity in fresh crude extracts from seedlings for ent-kaurene formation from geranyl-geranyl diphosphate, activity detected after some days of storage in liquid nitrogen, little effect of liquid nitrogen storage on EC 4.2.3.19 activity Helianthus annuus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
Helianthus annuus ent-kaurene + diphosphate
-
?
ent-copalyl diphosphate
-
Marah macrocarpa ent-kaurene + diphosphate
-
?
ent-copalyl diphosphate involved in biosynthesis of gibberellins Helianthus annuus ent-kaurene + diphosphate
-
?
ent-copalyl diphosphate involved in biosynthesis of gibberellins Marah macrocarpa ent-kaurene + diphosphate
-
?

Synonyms

Synonyms Comment Organism
More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Helianthus annuus
More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Marah macrocarpa