Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 as glutathione-S-transferase fusion protein, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity | Phaeosphaeria sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-isopropyl-4'(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate | Amo-1618 | Phaeosphaeria sp. | |
EDTA | - |
Phaeosphaeria sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Phaeosphaeria sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
132000 | - |
SDS-PAGE, cloned fusion protein | Phaeosphaeria sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | Phaeosphaeria sp. | involved in biosynthesis of gibberellins | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | Phaeosphaeria sp. L487 | involved in biosynthesis of gibberellins | ent-kaurene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaeosphaeria sp. | O13284 | - |
- |
Phaeosphaeria sp. L487 | O13284 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | - |
Phaeosphaeria sp. | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | involved in biosynthesis of gibberellins | Phaeosphaeria sp. | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Phaeosphaeria sp. L487 | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | involved in biosynthesis of gibberellins | Phaeosphaeria sp. L487 | ent-kaurene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) | Phaeosphaeria sp. |