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Literature summary for 4.2.3.19 extracted from

  • Kawaide, H.; Imai, R.; Sassa, T.; Kamiya, Y.
    ent-Kaurene synthetase from the fungus Phaeosphaeria sp. L487. cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase in fungal gibberellin biosynthesis (1997), J. Biol. Chem., 272, 21706-21712.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 as glutathione-S-transferase fusion protein, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity Phaeosphaeria sp.

Inhibitors

Inhibitors Comment Organism Structure
2'-isopropyl-4'(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate Amo-1618 Phaeosphaeria sp.
EDTA
-
Phaeosphaeria sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Phaeosphaeria sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
132000
-
SDS-PAGE, cloned fusion protein Phaeosphaeria sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Phaeosphaeria sp. involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
?
ent-copalyl diphosphate Phaeosphaeria sp. L487 involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Phaeosphaeria sp. O13284
-
-
Phaeosphaeria sp. L487 O13284
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
Phaeosphaeria sp. ent-kaurene + diphosphate
-
?
ent-copalyl diphosphate involved in biosynthesis of gibberellins Phaeosphaeria sp. ent-kaurene + diphosphate
-
?
ent-copalyl diphosphate
-
Phaeosphaeria sp. L487 ent-kaurene + diphosphate
-
?
ent-copalyl diphosphate involved in biosynthesis of gibberellins Phaeosphaeria sp. L487 ent-kaurene + diphosphate
-
?

Synonyms

Synonyms Comment Organism
More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Phaeosphaeria sp.