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Literature summary for 4.2.3.187 extracted from

  • Baer, P.; Rabe, P.; Citron, C.; De Oliveira Mann, C.; Kaufmann, N.; Groll, M.; Dickschat, J.
    Hedycaryol synthase in complex with nerolidol reveals terpene cyclase mechanism (2014), ChemBioChem, 15, 213-216 .
    View publication on PubMed
Search for more literature for 4.2.3.187

Cloned(Commentary)

Cloned (Comment) Organism
-
 Kitasatospora setae

Crystallization (Commentary)

Crystallization (Comment) Organism
structure in complex with nerolidol shows the key carbonyl oxygen of Val179, a highly conserved helix break (G1/2) and its corresponding helix dipole acting in . stabilisation of the carbocation at the substrate's C1 position and catalyzing 1,10 ring closure, thereby exclusively generating the anti-Markovnikov product Kitasatospora setae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O Kitasatospora setae
-
 (2Z,6E)-hedycaryol + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + H2O Kitasatospora setae DSM 43861
-
 (2Z,6E)-hedycaryol + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Kitasatospora setae E4MYY0
-
-
Kitasatospora setae DSM 43861 E4MYY0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
-
 Kitasatospora setae (2Z,6E)-hedycaryol + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + H2O
-
 Kitasatospora setae DSM 43861 (2Z,6E)-hedycaryol + diphosphate
-
 ?
additional information no substrates: geranyl diphosphate, (2Z,6E)-farnesyl diphosphate, (2E,6E,10E)-geranygeranyl diphosphate and (2Z,6E)-2-fluorofarnesyl diphosphate Kitasatospora setae ?
-
 ?
additional information no substrates: geranyl diphosphate, (2Z,6E)-farnesyl diphosphate, (2E,6E,10E)-geranygeranyl diphosphate and (2Z,6E)-2-fluorofarnesyl diphosphate Kitasatospora setae DSM 43861 ?
-
 ?

Synonyms

Synonyms Comment Organism
HCS
-
 Kitasatospora setae
KSE_00200t
-
 Kitasatospora setae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
41
-
 two-step transition, melting temperatures of 41°C and 49°C Kitasatospora setae
49
-
 two-step transition, melting temperatures of 41°C and 49°C Kitasatospora setae
52
-
 melting temperature in presence of 2-fluorofarnesyl diphosphate Kitasatospora setae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Kitasatospora setae