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Literature summary for 4.2.3.18 extracted from

  • Peters, R.J.; Croteau, R.B.
    Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate (2002), Biochemistry, 41, 1836-1842.
    View publication on PubMed
Search for more literature for 4.2.3.18

Protein Variants

Protein Variants Comment Organism
D361A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D402A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D402E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D402N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D404A
-
 Abies grandis
D404E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D404N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D405A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D405E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D405N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
E499A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
R365A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
R411A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
R454A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
W358A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
Y520A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis

Inhibitors

Inhibitors Comment Organism Structure
14,15-dihydro-15-aza-geranylgeranyl diphosphate
-
 Abies grandis
geranylgeranyl diphosphate
-
 Abies grandis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 copalyl diphosphate mutants D404N, R411A Abies grandis
0.0003
-
 copalyl diphosphate mutants D402A, D402N, D404E Abies grandis
0.0004
-
 copalyl diphosphate mutants W358A, D361A, D404A, D405E Abies grandis
0.0005
-
 copalyl diphosphate mutants D402E, D405E, R454A, E499A Y520A Abies grandis
0.0007
-
 copalyl diphosphate mutants D405N, R365A Abies grandis
132
-
 copalyl diphosphate
-
 Abies grandis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM selectively enables the conversion of copalyl diphosphate Abies grandis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-copalyl-diphosphate Abies grandis
-
 (-)-abietadiene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Abies grandis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-copalyl-diphosphate
-
 Abies grandis (-)-abietadiene + diphosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 copalyl diphosphate mutants D361A, D402A, D402E, D404N, D404A, D404E, D404N, D405A, D405E, D405N, D621A, E499A, R365A, R411A, R454A, W358A, Y520A Abies grandis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
 with copalyl diphosphate as substrate Abies grandis