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Literature summary for 4.2.3.178 extracted from

  • Matsuda, Y.; Mitsuhashi, T.; Quan, Z.; Abe, I.
    Molecular basis for stellatic acid biosynthesis a genome mining approach for discovery of sesterterpene synthases (2015), Org. Lett., 17, 4644-4647 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae and Escherichia coli Aspergillus stellatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the DDXXD motif for binding a trinuclear Mg2+ cluster is conserved in both the N-terminal terpene cyclase and C-terminal prenyl tranferase domains as 92DDVTD96 and 473DDVED477, respectively. Assay in presence of Mg2+ Aspergillus stellatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E,10E,14E)-geranylfarnesyl diphosphate Aspergillus stellatus
-
stellata-2,6,19-triene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus stellatus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E,10E,14E)-geranylfarnesyl diphosphate
-
Aspergillus stellatus stellata-2,6,19-triene + diphosphate
-
?

Synonyms

Synonyms Comment Organism
sesterterpene synthase
-
Aspergillus stellatus

General Information

General Information Comment Organism
physiological function the enzyme synthesizes the C25 linear substrate (2E,6E,10E,14E)-geranylfarnesyl diphosphate from dimethylallyl diphosphate and 4 molecules of isopentenyl dipohosphate and also cyclizes (2E,6E,10E,14E)-geranylfarnesyl diphosphate into stellata-2,6,19-triene, which is further transformed into stellatic acid by a cytochrome P450 monooxygenase encoded by the gene adjacent to the sesterterpene synthase gene Aspergillus stellatus