Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.17 extracted from

  • Williams, D.C.; Wildung, M.R.; Jin, A.Q.; Dalal, D.; Oliver, J.S.; Coates, R.M.; Croteau, R.
    Heterologous xpression and characterization of a "Pseudomature" form of taxadiene synthase involved in paclitaxel (taxol) biosynthesis and evaluation of a potential intermediate and inhibitors of the multistep diterpene cyclization reaction (2000), Arch. Biochem. Biophys., 379, 137-146.
    View publication on PubMed
Search for more literature for 4.2.3.17

Application

Application Comment Organism
medicine chemotherapeutic agent against a range of cancers, including ovarian and breast cancer Taxus brevifolia

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Taxus brevifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 geranylgeranyl diphosphate native enzyme Taxus brevifolia
0.0031
-
 geranylgeranyl diphosphate thioredoxin fusion Taxus brevifolia
0.0105
-
 geranylgeranyl diphosphate M1 preprotein Taxus brevifolia
0.014
-
 geranylgeranyl diphosphate M93 Taxus brevifolia
0.0159
-
 geranylgeranyl diphosphate M79 Taxus brevifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Taxus brevifolia catalyzes the first step in taxol biosynthesis taxa-4,11-diene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus brevifolia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Taxus brevifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclopropylidene
-
 Taxus brevifolia ?
-
 ?
geranylgeranyl diphosphate
-
 Taxus brevifolia taxa-4(5),11(12)-diene + diphosphate
-
 ?
geranylgeranyl diphosphate taxa-4(20),11(12)-diene is formed as a small amount Taxus brevifolia taxa-4(20),11(12)-diene + diphosphate
-
 ?
geranylgeranyl diphosphate catalyzes the first step in taxol biosynthesis Taxus brevifolia taxa-4,11-diene + diphosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00004
-
 geranylgeranyl diphosphate M1 preprotein Taxus brevifolia
0.0000417
-
 geranylgeranyl diphosphate thioredoxin fusion Taxus brevifolia
0.000133
-
 geranylgeranyl diphosphate M93 Taxus brevifolia
0.00017
-
 geranylgeranyl diphosphate M79 Taxus brevifolia
0.000177
-
 geranylgeranyl diphosphate native enzyme Taxus brevifolia