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Literature summary for 4.2.3.165 extracted from

  • Burkhardt, I.; Siemon, T.; Henrot, M.; Studt, L.; Rosler, S.; Tudzynski, B.; Christmann, M.; Dickschat, J.S.
    Mechanistic Characterisation of two sesquiterpene cyclases from the plant pathogenic fungus Fusarium fujikuroi (2016), Angew. Chem. Int. Ed. Engl., 55, 8748-8751.
    View publication on PubMed
Search for more literature for 4.2.3.165

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Fusarium proliferatum
overexpression in Escherichia coli Fusarium mangiferae
overexpression in Escherichia coli Fusarium fujikuroi

Protein Variants

Protein Variants Comment Organism
K288N the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis restores activity Fusarium fujikuroi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Fusarium proliferatum
-
 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate Fusarium mangiferae
-
 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate Fusarium fujikuroi
-
 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate Fusarium fujikuroi IMI 58289
-
 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium fujikuroi S0ENM8 the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity
-
Fusarium fujikuroi IMI 58289 S0ENM8 the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity
-
Fusarium mangiferae
-
-
-
Fusarium proliferatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fusarium proliferatum
-
 Fusarium mangiferae
-
 Fusarium fujikuroi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Fusarium proliferatum (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Fusarium mangiferae (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Fusarium fujikuroi (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate no activity with geranyl diphosphate and geranylgeranyl diphosphate Fusarium proliferatum (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene i.e. (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene ?
(2E,6E)-farnesyl diphosphate no activity with geranyl diphosphate and geranylgeranyl diphosphate Fusarium mangiferae (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene i.e. (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene ?
(2E,6E)-farnesyl diphosphate the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity. No activity with geranyl diphosphate and geranylgeranyl diphosphate Fusarium fujikuroi (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene = (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene ?
(2E,6E)-farnesyl diphosphate
-
 Fusarium fujikuroi IMI 58289 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity. No activity with geranyl diphosphate and geranylgeranyl diphosphate Fusarium fujikuroi IMI 58289 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene = (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene ?

Synonyms

Synonyms Comment Organism
STC5
-
 Fusarium proliferatum
STC5
-
 Fusarium mangiferae
STC5
-
 Fusarium fujikuroi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay Fusarium proliferatum
28
-
 assay Fusarium mangiferae
28
-
 assay Fusarium fujikuroi