Literature summary for 4.2.3.165 extracted from

  • Burkhardt, I.; Siemon, T.; Henrot, M.; Studt, L.; Rosler, S.; Tudzynski, B.; Christmann, M.; Dickschat, J.S.
    Mechanistic Characterisation of two sesquiterpene cyclases from the plant pathogenic fungus Fusarium fujikuroi (2016), Angew. Chem. Int. Ed. Engl., 55, 8748-8751.
    View publication on PubMed

Cloned(Commentary)

Cloned (Commentary) Organism
overexpression in Escherichia coli Fusarium proliferatum
overexpression in Escherichia coli Fusarium mangiferae
overexpression in Escherichia coli Fusarium fujikuroi

Engineering

Protein Variants Commentary Organism
K288N the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis restores activity Fusarium fujikuroi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility ID
(2E,6E)-farnesyl diphosphate Fusarium proliferatum
-
(1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
?
(2E,6E)-farnesyl diphosphate Fusarium mangiferae
-
(1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
?
(2E,6E)-farnesyl diphosphate Fusarium fujikuroi
-
(1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
?
(2E,6E)-farnesyl diphosphate Fusarium fujikuroi IMI 58289
-
(1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
?

Organism

Organism UniProt Commentary Textmining
Fusarium proliferatum
-
-
-
Fusarium mangiferae
-
-
-
Fusarium fujikuroi S0ENM8 the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity
-
Fusarium fujikuroi IMI 58289 S0ENM8 the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity
-

Purification (Commentary)

Purification (Commentary) Organism
-
Fusarium proliferatum
-
Fusarium mangiferae
-
Fusarium fujikuroi

Substrates and Products (Substrate)

Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility Substrate Product ID
(2E,6E)-farnesyl diphosphate
-
739857 Fusarium proliferatum (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate
-
739857 Fusarium mangiferae (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate
-
739857 Fusarium fujikuroi (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate no activity with geranyl diphosphate and geranylgeranyl diphosphate 739857 Fusarium proliferatum (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene i.e. (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene
-
-
?
(2E,6E)-farnesyl diphosphate no activity with geranyl diphosphate and geranylgeranyl diphosphate 739857 Fusarium mangiferae (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene i.e. (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene
-
-
?
(2E,6E)-farnesyl diphosphate the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity. No activity with geranyl diphosphate and geranylgeranyl diphosphate 739857 Fusarium fujikuroi (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene = (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene
-
-
?
(2E,6E)-farnesyl diphosphate
-
739857 Fusarium fujikuroi IMI 58289 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity. No activity with geranyl diphosphate and geranylgeranyl diphosphate 739857 Fusarium fujikuroi IMI 58289 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene = (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene
-
-
?

Synonyms

Synonyms Commentary Organism
STC5
-
Fusarium proliferatum
STC5
-
Fusarium mangiferae
STC5
-
Fusarium fujikuroi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
28
-
assay Fusarium proliferatum
28
-
assay Fusarium mangiferae
28
-
assay Fusarium fujikuroi

Cloned(Commentary) (protein specific)

Commentary Organism
overexpression in Escherichia coli Fusarium proliferatum
overexpression in Escherichia coli Fusarium mangiferae
overexpression in Escherichia coli Fusarium fujikuroi

Engineering (protein specific)

Protein Variants Commentary Organism
K288N the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis restores activity Fusarium fujikuroi

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility ID
(2E,6E)-farnesyl diphosphate Fusarium proliferatum
-
(1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
?
(2E,6E)-farnesyl diphosphate Fusarium mangiferae
-
(1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
?
(2E,6E)-farnesyl diphosphate Fusarium fujikuroi
-
(1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
?
(2E,6E)-farnesyl diphosphate Fusarium fujikuroi IMI 58289
-
(1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
?

Purification (Commentary) (protein specific)

Commentary Organism
-
Fusarium proliferatum
-
Fusarium mangiferae
-
Fusarium fujikuroi

Substrates and Products (Substrate) (protein specific)

Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility ID
(2E,6E)-farnesyl diphosphate
-
739857 Fusarium proliferatum (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate
-
739857 Fusarium mangiferae (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate
-
739857 Fusarium fujikuroi (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate no activity with geranyl diphosphate and geranylgeranyl diphosphate 739857 Fusarium proliferatum (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene i.e. (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene
-
-
?
(2E,6E)-farnesyl diphosphate no activity with geranyl diphosphate and geranylgeranyl diphosphate 739857 Fusarium mangiferae (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene i.e. (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene
-
-
?
(2E,6E)-farnesyl diphosphate the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity. No activity with geranyl diphosphate and geranylgeranyl diphosphate 739857 Fusarium fujikuroi (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene = (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene
-
-
?
(2E,6E)-farnesyl diphosphate
-
739857 Fusarium fujikuroi IMI 58289 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity. No activity with geranyl diphosphate and geranylgeranyl diphosphate 739857 Fusarium fujikuroi IMI 58289 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene = (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene
-
-
?

Temperature Optimum [°C] (protein specific)

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
28
-
assay Fusarium proliferatum
28
-
assay Fusarium mangiferae
28
-
assay Fusarium fujikuroi