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Literature summary for 4.2.3.151 extracted from

  • Meguro, A.; Tomita, T.; Nishiyama, M.; Kuzuyama, T.
    Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton (2013), ChemBioChem, 14, 316-321.
    View publication on PubMed
Search for more literature for 4.2.3.151

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0421
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Streptomyces sp.
69000
-
 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Streptomyces sp.
-
 (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?
geranylgeranyl diphosphate Streptomyces sp. SANK 60404
-
 (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. M1VDX3
-
-
Streptomyces sp. SANK 60404 M1VDX3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
 Streptomyces sp. (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?
geranylgeranyl diphosphate the trifunctional enzyme also produces (R)-nephthenol and (R)-cembrene A. See EC 4.2.3.150, cembrene A synthase and EC 4.2.3.149, nephthenol synthase Streptomyces sp. (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?
geranylgeranyl diphosphate
-
 Streptomyces sp. SANK 60404 (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
DtcycB gene name. The trifunctional enzyme also produces (R)-nephthenol and (R)-cembrene A. See EC 4.2.3.150, cembrene A synthase and EC 4.2.3.149, nephthenol synthase Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.022
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay Streptomyces sp.