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Literature summary for 4.2.3.15 extracted from

  • Ruan, J.X.; Li, J.X.; Fang, X.; Wang, L.J.; Hu, W.L.; Chen, X.Y.; Yang, C.Q.
    Isolation and characterization of three new monoterpene synthases from Artemisia annua (2016), Front. Plant Sci., 7, 638 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.15

Cloned(Commentary)

Cloned (Comment) Organism
gene TPS2, cloning from a cDNA library, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme without after removing the N-terminal plastid targeting sequence in Escherichia coli strain BL21(DE3) Artemisia annua

Protein Variants

Protein Variants Comment Organism
additional information the N-terminal signal peptide of AaTPS2 (46 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) Artemisia annua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Artemisia annua
0.00825
-
 geranyl diphosphate pH 7.0, 30°C, recombinant enzyme Artemisia annua

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, Mg2+ is the highly preferred divalent cation Artemisia annua
Mn2+ low activity Artemisia annua
additional information although both Mg2+ and Mn2+ are able to support the catalytic activity of enzyme AaTPS2, an altered product spectrum is observed in the presence of Mn2+ for AaTPS2 Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate Artemisia annua
-
 beta-myrcene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Artemisia annua A0A068L9J0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme without N-terminal signal peptide from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Artemisia annua

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Artemisia annua
-
additional information quantitative RT-PCR expression analysis in plants tissues. AaTPS2 exhibits the highest expression level in stem, and low expression in the other tissues Artemisia annua
-
root
-
 Artemisia annua
-
stem highest expression level of AaTPS2 Artemisia annua
-
trichome glandular Artemisia annua
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate
-
 Artemisia annua beta-myrcene + diphosphate
-
 ?
additional information GC-MS analysis and identification of products from enzyme catalysis. Enzyme AaTPS2 produces a single product, beta-myrcene. No activity with farnesyl diphosphate or geranylgeranyl diphosphate Artemisia annua ?
-
 ?

Synonyms

Synonyms Comment Organism
AaTPS2
-
 Artemisia annua
TPS2
-
 Artemisia annua

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Artemisia annua

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
 geranyl diphosphate pH 7.0, 30°C, recombinant enzyme Artemisia annua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Artemisia annua

Expression

Organism Comment Expression
Artemisia annua AaTPS2 is upregulated after mechanical wounding, but treatments with phytohormones (salicylic acid, methyl jasmonate, and gibberellin) does not affect the expression of AaTPS2 up

General Information

General Information Comment Organism
evolution the enzyme AaTPS2 belongs to the TPS superfamily, subclade TPS-b Artemisia annua
metabolism in Artemisia annua, three monoterpene synthases AaTPS2, AaTPS5, and AaTPS6, produce beta-myrcene, camphene, and 1,8-cineole as the major products, respectively, analysis of monoterpenes and sesquiterpenes produced by Artemisia annua plants, overview Artemisia annua

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
63.03
-
 geranyl diphosphate pH 7.0, 30°C, recombinant enzyme Artemisia annua