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Literature summary for 4.2.3.15 extracted from

  • Green, S.; Squire, C.J.; Nieuwenhuizen, N.J.; Baker, E.N.; Laing, W.
    Defining the potassium binding region in an apple terpene synthase (2009), J. Biol. Chem., 284, 8661-8669.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
mutated enzymes are generated using the QuickChange II site-directed mutagenesis kit. The PCR-based mutagenesis protocol is performed using pET200-D-TOPO harbouring the respective AcTPS2 cDNAs as template. The single-site mutant enzymes overexpressed in Escherichia coli. Actinidia chinensis

Protein Variants

Protein Variants Comment Organism
K514A AcTPS2 mutant, 90% reduction in monoterpene synthase activity compared to wild-type enzyme Actinidia chinensis
K514S AcTPS2 mutant, presence of Ser is not able to rescue any of the activity lost after the removal of the Lys residue Actinidia chinensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM are included in farnesyl diphosphate activity assay Actinidia chinensis
additional information wild-type AcTPS2 is significantly more active when KCl is absent from the activity buffer Actinidia chinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Actinidia chinensis terpenes produced from the wild-type and mutant enzymes are identical, showing low levels of beta-myrcene from AcTPS2-K514A ?
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?

Organism

Organism UniProt Comment Textmining
Actinidia chinensis
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AcTPS2 protein is extracted and purified Actinidia chinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information terpenes produced from the wild-type and mutant enzymes are identical, showing low levels of beta-myrcene from AcTPS2-K514A Actinidia chinensis ?
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?

Synonyms

Synonyms Comment Organism
AcTPS2
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Actinidia chinensis
myrcene synthase
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Actinidia chinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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farnesyl diphosphate and geranyl diphosphate activity assay Actinidia chinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
farnesyl diphosphate activity assay Actinidia chinensis