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Literature summary for 4.2.3.144 extracted from

  • Falara, V.; Alba, J.; Kant, M.; Schuurink, R.; Pichersky, E.
    Geranyllinalool synthases in Solanaceae and other angiosperms constitute an ancient branch of diterpene synthases involved in the synthesis of defensive compounds (2014), Plant Physiol., 166, 428-441 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene NaTPS46, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli Nicotiana attenuata
gene SlTPS46 encoding SlGLS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His-tagged SlTPS46 in Escherichia coli Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0187
-
geranylgeranyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum
0.0315
-
geranylgeranyl diphosphate pH and temperature not specified in the publication Nicotiana attenuata
0.0637
-
(2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O Solanum lycopersicum reaction of EC 4.2.3.48 (3S,6E)-nerolidol + diphosphate
-
?
geranylgeranyl diphosphate + H2O Solanum lycopersicum
-
(6E,10E)-geranyllinalool + diphosphate
-
?
geranylgeranyl diphosphate + H2O Nicotiana attenuata
-
(6E,10E)-geranyllinalool + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana attenuata A0A088DC07
-
-
Solanum lycopersicum K4BE78
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged SlTPS46 from Escherichia coli by nickel affinity chromatography Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Solanum lycopersicum
-
fruit green and red Solanum lycopersicum
-
leaf
-
Nicotiana attenuata
-
leaf expression is higher in young leaves compared to old ones Solanum lycopersicum
-
root
-
Solanum lycopersicum
-
stem
-
Solanum lycopersicum
-
trichome
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O reaction of EC 4.2.3.48 Solanum lycopersicum (3S,6E)-nerolidol + diphosphate
-
?
geranylgeranyl diphosphate + H2O
-
Solanum lycopersicum (6E,10E)-geranyllinalool + diphosphate
-
?
geranylgeranyl diphosphate + H2O
-
Nicotiana attenuata (6E,10E)-geranyllinalool + diphosphate
-
?
geranylgeranyl diphosphate + H2O preferred substrate Solanum lycopersicum (6E,10E)-geranyllinalool + diphosphate
-
?
additional information enzyme NaGLS is not active with geranyl diphosphate and farnesyl diphosphate, GC-MS analysis of compounds Nicotiana attenuata ?
-
?
additional information enzyme SlTPS46 shows no activity with geranyl diphospate, GC-MS analysis of compounds Solanum lycopersicum ?
-
?

Synonyms

Synonyms Comment Organism
101262489 gene name, UniProt Solanum lycopersicum
geranyllinalool synthase
-
Solanum lycopersicum
geranyllinalool synthase
-
Nicotiana attenuata
GES gene name, UniProt Nicotiana attenuata
GLS
-
Solanum lycopersicum
GLS
-
Nicotiana attenuata
More cf. EC 4.2.3.48 Solanum lycopersicum
NaGLS
-
Nicotiana attenuata
SlGLS
-
Solanum lycopersicum
SlTPS46
-
Solanum lycopersicum
TPS-e/f
-
Solanum lycopersicum
TPS-e/f
-
Nicotiana attenuata
TPS46
-
Solanum lycopersicum
TPS46
-
Nicotiana attenuata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.43
-
(2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum
6.85
-
geranylgeranyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum
7.53
-
geranylgeranyl diphosphate pH and temperature not specified in the publication Nicotiana attenuata

Expression

Organism Comment Expression
Solanum lycopersicum suppression of the enzyme by methyl salicylate down
Solanum lycopersicum no induction of the enzyme by the defense-suppressing mite line Tetranycus evansi Vicosa-1 additional information
Nicotiana attenuata NaGLS is expressed constitutively, but the gene NaTPS46 can be induced in leaves with methyl jasmonate up
Solanum lycopersicum TPS46 shows induction by alamethicin and methyljasmonate treatment, induction by defense-inducing spider mite line Tetranycus urticae Santpoort-2 up

General Information

General Information Comment Organism
evolution SlGLS, NaGLS, AtGLSs, and several other GLSs come from different eudicot families and constitute a separate branch of the TPS-e/f clade that diverged from kaurene synthases, also in the TPS-e/f clade, before the gymnosperm-angiosperm split. The early divergence of this branch and the GLS activity of genes in this branch in diverse eudicot families suggest that GLS activity encoded by these genes predates the angiosperm-gymnosperm split Solanum lycopersicum
evolution SlGLS, NaGLS, AtGLSs, and several other GLSs come from different eudicot families and constitute a separate branch of the TPS-e/f clade that diverged from kaurene synthases, also in the TPS-e/f clade, before the gymnosperm-angiosperm split. The early divergence of this branch and the GLS activity of genes in this branch in diverse eudicot families suggest that GLS activity encoded by these genes predates the angiosperm-gymnosperm split Nicotiana attenuata
metabolism the terpene synthase (TPS) catalyzes a two-step reaction in which carbocation formation of the C20 precursor geranylgeranyl diphosphate (GGPP) is followed by an allylic rearrangement that results in the production of the tertiary alcohol geranyllinalool, which is the precursor for biosynthesis of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene, TMTT Solanum lycopersicum
metabolism the terpene synthase (TPS) catalyzes a two-step reaction in which carbocation formation of the C20 precursor geranylgeranyl diphosphate (GGPP) is followed by an allylic rearrangement that results in the production of the tertiary alcohol geranyllinalool, which is the precursor for biosynthesis of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene, TMTT Nicotiana attenuata
physiological function GC-MS analysis of terpene volatiles induced by alamethicin treatment, overview Solanum lycopersicum
physiological function GC-MS analysis of terpene volatiles induced by alamethicin treatment, overview Nicotiana attenuata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.75
-
(2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum
239.05
-
geranylgeranyl diphosphate pH and temperature not specified in the publication Nicotiana attenuata
366.3
-
geranylgeranyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum