Cloned (Comment) | Organism |
---|---|
gene NaTPS46, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli | Nicotiana attenuata |
gene SlTPS46 encoding SlGLS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His-tagged SlTPS46 in Escherichia coli | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0187 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Solanum lycopersicum | |
0.0315 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Nicotiana attenuata | |
0.0637 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | Solanum lycopersicum | reaction of EC 4.2.3.48 | (3S,6E)-nerolidol + diphosphate | - |
? | |
geranylgeranyl diphosphate + H2O | Solanum lycopersicum | - |
(6E,10E)-geranyllinalool + diphosphate | - |
? | |
geranylgeranyl diphosphate + H2O | Nicotiana attenuata | - |
(6E,10E)-geranyllinalool + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana attenuata | A0A088DC07 | - |
- |
Solanum lycopersicum | K4BE78 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged SlTPS46 from Escherichia coli by nickel affinity chromatography | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Solanum lycopersicum | - |
fruit | green and red | Solanum lycopersicum | - |
leaf | - |
Nicotiana attenuata | - |
leaf | expression is higher in young leaves compared to old ones | Solanum lycopersicum | - |
root | - |
Solanum lycopersicum | - |
stem | - |
Solanum lycopersicum | - |
trichome | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | reaction of EC 4.2.3.48 | Solanum lycopersicum | (3S,6E)-nerolidol + diphosphate | - |
? | |
geranylgeranyl diphosphate + H2O | - |
Solanum lycopersicum | (6E,10E)-geranyllinalool + diphosphate | - |
? | |
geranylgeranyl diphosphate + H2O | - |
Nicotiana attenuata | (6E,10E)-geranyllinalool + diphosphate | - |
? | |
geranylgeranyl diphosphate + H2O | preferred substrate | Solanum lycopersicum | (6E,10E)-geranyllinalool + diphosphate | - |
? | |
additional information | enzyme NaGLS is not active with geranyl diphosphate and farnesyl diphosphate, GC-MS analysis of compounds | Nicotiana attenuata | ? | - |
? | |
additional information | enzyme SlTPS46 shows no activity with geranyl diphospate, GC-MS analysis of compounds | Solanum lycopersicum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
101262489 | gene name, UniProt | Solanum lycopersicum |
geranyllinalool synthase | - |
Solanum lycopersicum |
geranyllinalool synthase | - |
Nicotiana attenuata |
GES | gene name, UniProt | Nicotiana attenuata |
GLS | - |
Solanum lycopersicum |
GLS | - |
Nicotiana attenuata |
More | cf. EC 4.2.3.48 | Solanum lycopersicum |
NaGLS | - |
Nicotiana attenuata |
SlGLS | - |
Solanum lycopersicum |
SlTPS46 | - |
Solanum lycopersicum |
TPS-e/f | - |
Solanum lycopersicum |
TPS-e/f | - |
Nicotiana attenuata |
TPS46 | - |
Solanum lycopersicum |
TPS46 | - |
Nicotiana attenuata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Solanum lycopersicum | |
6.85 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Solanum lycopersicum | |
7.53 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Nicotiana attenuata |
Organism | Comment | Expression |
---|---|---|
Solanum lycopersicum | suppression of the enzyme by methyl salicylate | down |
Solanum lycopersicum | no induction of the enzyme by the defense-suppressing mite line Tetranycus evansi Vicosa-1 | additional information |
Nicotiana attenuata | NaGLS is expressed constitutively, but the gene NaTPS46 can be induced in leaves with methyl jasmonate | up |
Solanum lycopersicum | TPS46 shows induction by alamethicin and methyljasmonate treatment, induction by defense-inducing spider mite line Tetranycus urticae Santpoort-2 | up |
General Information | Comment | Organism |
---|---|---|
evolution | SlGLS, NaGLS, AtGLSs, and several other GLSs come from different eudicot families and constitute a separate branch of the TPS-e/f clade that diverged from kaurene synthases, also in the TPS-e/f clade, before the gymnosperm-angiosperm split. The early divergence of this branch and the GLS activity of genes in this branch in diverse eudicot families suggest that GLS activity encoded by these genes predates the angiosperm-gymnosperm split | Solanum lycopersicum |
evolution | SlGLS, NaGLS, AtGLSs, and several other GLSs come from different eudicot families and constitute a separate branch of the TPS-e/f clade that diverged from kaurene synthases, also in the TPS-e/f clade, before the gymnosperm-angiosperm split. The early divergence of this branch and the GLS activity of genes in this branch in diverse eudicot families suggest that GLS activity encoded by these genes predates the angiosperm-gymnosperm split | Nicotiana attenuata |
metabolism | the terpene synthase (TPS) catalyzes a two-step reaction in which carbocation formation of the C20 precursor geranylgeranyl diphosphate (GGPP) is followed by an allylic rearrangement that results in the production of the tertiary alcohol geranyllinalool, which is the precursor for biosynthesis of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene, TMTT | Solanum lycopersicum |
metabolism | the terpene synthase (TPS) catalyzes a two-step reaction in which carbocation formation of the C20 precursor geranylgeranyl diphosphate (GGPP) is followed by an allylic rearrangement that results in the production of the tertiary alcohol geranyllinalool, which is the precursor for biosynthesis of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene, TMTT | Nicotiana attenuata |
physiological function | GC-MS analysis of terpene volatiles induced by alamethicin treatment, overview | Solanum lycopersicum |
physiological function | GC-MS analysis of terpene volatiles induced by alamethicin treatment, overview | Nicotiana attenuata |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.75 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Solanum lycopersicum | |
239.05 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Nicotiana attenuata | |
366.3 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Solanum lycopersicum |