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Literature summary for 4.2.3.135 extracted from
- Insight into metabolic diversity of the brown-rot basidiomycete Postia placenta responsible for sesquiterpene biosynthesis semi-comprehensive screening of cytochrome P450 monooxygenase involved in protoilludene metabolism (2018), Microb. Biotechnol., 11, 952-965 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PpSTS-08, coexpression of protoilludene synthase PpSTS-08 and diverse PpCYPs in Saccharomyces cerevisiae by chromosomal integration of the PpSTS-08 expression cassette and subsequent transformation with expression plasmids of 184 PpCYPs previously constructed resulting in 184 distinct transformants that give simultaneous expression of PpSTS-08 with each of the PpCYPs | Postia placenta |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | coexpression of protoilludene synthase PpSTS-08 and diverse PpCYPs in Saccharomyces cerevisiae by chromosomal integration of the PpSTS-08 expression cassette and subsequent transformation with expression plasmids of 184 PpCYPs previously constructed resulting in 184 distinct transformants that give simultaneous expression of PpSTS-08 with each of the PpCYPs | Postia placenta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | Postia placenta | - |
DELTA6-protoilludene + diphosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Postia placenta | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Postia placenta | DELTA6-protoilludene + diphosphate | - |
? | |
| additional information | PpSTS-08 product formation is identified by GC-MS and NMR analysis | Postia placenta | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PpSTS-08 | - |
Postia placenta |
| protoilludene synthase | - |
Postia placenta |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the three cytochrome P450 monooxygenases CYP5344B1, CYP5348E1, and CYP5348J3 show substantial activities against DELTA6-protoilludene catalyzing the hydroxylation reaction of DELTA6-protoilludene to produce DELTA6-protoilludene-8-ol and DELTA6-protoilludene-5-ol | Postia placenta |
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Release 2023.1 (January 2023)
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