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Literature summary for 4.2.3.135 extracted from

  • Quin, M.; Flynn, C.; Wawrzyn, G.; Choudhary, S.; Schmidt-Dannert, C.
    Mushroom hunting by using bioinformatics: Application of a predictive framework facilitates the selective identification of sesquiterpene synthases in Basidiomycota (2013), ChemBioChem, 14, 2480-2491.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.135

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00152
-
 (2E,6E)-farnesyl diphosphate isoform 73029, pH 8.0, 21°C Stereum hirsutum
0.00191
-
 (2E,6E)-farnesyl diphosphate isoform 64702, pH 8.0, 21°C Stereum hirsutum
0.00502
-
 (2E,6E)-farnesyl diphosphate isoform 25180, pH 8.0, 21°C Stereum hirsutum

Organism

Organism UniProt Comment Textmining
Stereum hirsutum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(2E,6E)-farnesyl diphosphate = DELTA6-protoilludene + diphosphate 1,11-ring closure of (2E,6E)-farnsyl diphosphate yields a highly reactive trans-humulyl carbocation. A 1,2-hydride shift followed by further ring closures results in the protoilludyl cation, which undergoes a final deprotonation to give DELTA6-protoilludene Stereum hirsutum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Stereum hirsutum DELTA6-protoilludene + diphosphate main product for isoforms 25180, 64702, 73029. Isoforms 64702 and 73029 appear to be less specific than other previously described DELTA6-protoilludene synthases, and are capable of simultaneously producing a 1,10-cyclization-derived scaffold, beta-elemene, when expressed in a heterologous host ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0037
-
 (2E,6E)-farnesyl diphosphate isoform 64702, pH 8.0, 21°C Stereum hirsutum
0.0044
-
 (2E,6E)-farnesyl diphosphate isoform 25180, pH 8.0, 21°C Stereum hirsutum
0.0063
-
 (2E,6E)-farnesyl diphosphate isoform 73029, pH 8.0, 21°C Stereum hirsutum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.889
-
 (2E,6E)-farnesyl diphosphate isoform 25180, pH 8.0, 21°C Stereum hirsutum
1.951
-
 (2E,6E)-farnesyl diphosphate isoform 64702, pH 8.0, 21°C Stereum hirsutum
4.284
-
 (2E,6E)-farnesyl diphosphate isoform 73029, pH 8.0, 21°C Stereum hirsutum