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Literature summary for 4.2.3.135 extracted from
- Cloning and characterization of an Armillaria gallica cDNA encoding protoilludene synthase, which catalyzes the first committed step in the synthesis of antimicrobial melleolides (2011), J. Biol. Chem., 286, 6871-6878.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Armillaria gallica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | 5% ethanol causes strongly reduced activity | Armillaria gallica |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00053 | - |
(2E,6E)farnesyl diphosphate | pH 7.2, 22°C | Armillaria gallica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | highest activity in the presence of 5 mM MgCl2 | Armillaria gallica | |
| MnCl2 | inhibits activity | Armillaria gallica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
calculated from cDNA | Armillaria gallica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Armillaria gallica | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)farnesyl diphosphate | - |
Armillaria gallica | 6-protoilludene + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| protoilludene synthase | - |
Armillaria gallica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
- |
Armillaria gallica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
nearly complete loss of activity above 35°C | Armillaria gallica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Armillaria gallica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
only 50% activity | Armillaria gallica |
| 8.5 | - |
only 50% activity | Armillaria gallica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | 6-protoilludene is the exclusive reaction product of protoilludene synthase | Armillaria gallica |
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Release 2023.1 (January 2023)
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