Literature summary for 4.2.3.131 extracted from

Bozic, D.; Papaefthimiou, D.; Brueckner, K.; de Vos, R.C.; Tsoleridis, C.A.; Katsarou, D.; Papanikolaou, A.; Pateraki, I.; Chatzopoulou, F.M.; Dimitriadou, E.; Kostas, S.; Manzano, D.; Scheler, U.; Ferrer, A.; Tissier, A.; Makris, A.M.; Kampranis, S.C.; Kanellis, A.K.
Towards elucidating carnosic acid biosynthesis in Lamiaceae Functional characterization of the three first steps of the pathway in Salvia fruticosa and Rosmarinus officinalis (2015), PLoS ONE, 10, e0124106 .

Search for more literature for 4.2.3.131

Cloned(Commentary)

Cloned (Comment)	Organism
gene KSL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, real-time PCR enzyme expression analysis, recombinant expression of HA-His-tandem-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL, functional transient recombinant coexpression of Salvia fruticosa (+)-copalyl diphosphate sythase (SfCPS) and Salvia fruticosa ent-kaurene synthase-like (SfKSL) in Nicotiana benthamiana via transfection by Agrobacterium tumefaciens leading to production of miltiradiene in planta, NMR product analysis	Salvia fruticosa

Cloned (Comment)

Organism

gene KSL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, real-time PCR enzyme expression analysis, recombinant expression of HA-His-tandem-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL, functional transient recombinant coexpression of Salvia fruticosa (+)-copalyl diphosphate sythase (SfCPS) and Salvia fruticosa ent-kaurene synthase-like (SfKSL) in Nicotiana benthamiana via transfection by Agrobacterium tumefaciens leading to production of miltiradiene in planta, NMR product analysis

Salvia fruticosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Salvia fruticosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(+)-copalyl diphosphate	Salvia fruticosa	-	miltiradiene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Salvia fruticosa	A0A0C5QH30	genotypes France, Vrysses, and Kavoussi	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant HA-His-tandem-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography	Salvia fruticosa

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	young leaves show higher accumulation of SfKSL transcripts, when compared to the fully expanded leaves, consistent with the trichome and developmental regulation of carnosic acid build-up	Salvia fruticosa	-
additional information	real-time PCR analysis demonstrates higher expression level of SfKSL in isolated trichomes than in leaves without trichomes	Salvia fruticosa	-
trichome	glandular, transcriptome analysis, overview	Salvia fruticosa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-copalyl diphosphate	-	Salvia fruticosa	miltiradiene + diphosphate	-	?
additional information	synthesis of miltiradiene through the enzyme is confirmed by 1D and 2D NMR analyses, GC-MS analysis, overview	Salvia fruticosa	?	-	?

Synonyms

Synonyms	Comment	Organism
ent-kaurene synthase-like	-	Salvia fruticosa
KSL	-	Salvia fruticosa
SfKSL	-	Salvia fruticosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Salvia fruticosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Salvia fruticosa

General Information

General Information	Comment	Organism
physiological function	enzyme SfKSL is responsible for the formation of the carnosic acid precursor miltiradiene	Salvia fruticosa