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Literature summary for 4.2.3.13 extracted from
- Cloning and heterologous expression of a second (+)-delta-cadinene synthase from Gossypium arboreum (1996), J. Nat. Prod., 59, 944-951.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli of the CAD1-A isoenzyme, 80% of homology with CAD1-C isoenzyme | Gossypium arboreum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
2-trans,6-trans-farnesyl diphosphate | isoenzyme CAD1-A, pH 8.7, 30°C | Gossypium arboreum |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2.5 mM MgCl2 required for optimal activity, enzyme form CAD1-A | Gossypium arboreum | |
| Mg2+ | 15 mM MgCl2 required for optimal activity, enzyme form CAD1-C | Gossypium arboreum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64060 | - |
calculated from DNA sequence, CAD1-A isozyme | Gossypium arboreum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gossypium arboreum | - |
cv. Nanking | - |
| Gossypium arboreum | - |
two enzyme forms CAD1-A and CAD1-C1 | - |
| Gossypium arboreum | - |
tree cotton | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CAD1-A isoenzyme | Gossypium arboreum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.021 | - |
CAD1-A isozyme, expressed in Escherichia coli | Gossypium arboreum |
| 0.036 | - |
CAD1-A isozyme, removal of 15 amino acids by thrombin cleavage | Gossypium arboreum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-trans,6-trans-farnesyl diphosphate | - |
Gossypium arboreum | (+)-delta-cadinene + diphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | enzyme form CAD1-C | Gossypium arboreum |
| 8.7 | - |
enzyme form CAD1-A | Gossypium arboreum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.7 | 62% of maximal activity at pH 6.8, enzyme form CAD1-A | Gossypium arboreum |
| 7 | 8.7 | 60% of maximal activity at pH 8.7, enzyme form CAD1-C | Gossypium arboreum |
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