BRENDA - Enzyme Database
show all sequences of 4.2.3.127

The floral transcriptome of ylang ylang (Cananga odorata var. fruticosa) uncovers biosynthetic pathways for volatile organic compounds and a multifunctional and novel sesquiterpene synthase

Jin, J.; Kim, M.J.; Dhandapani, S.; Tjhang, J.G.; Yin, J.L.; Wong, L.; Sarojam, R.; Chua, N.H.; Jang, I.C.; J. Exp. Bot. 66, 3959-3975 (2015) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene TPS2, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, transient and functional recombinant expression of YFP-tagged enzyme CoTPS2 in transgenic Nicotiana benthamiana leaves in cytosol under control of CaMV 35S promoter via Agrobacterium tumefaciens-mediated transfection method
Cananga odorata var. fruticosa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Cananga odorata var. fruticosa
5829
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, enzyme CoTPS2 has the conserved aspartate-rich motif (DDXXD) and NSE/DTE motifs that chelate divalent metal ions, typically Mg2+, in the C-terminal domain
Cananga odorata var. fruticosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2E,6E)-farnesyl diphosphate
Cananga odorata var. fruticosa
-
beta-copaene + diphosphate
-
-
?
additional information
Cananga odorata var. fruticosa
enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Cananga odorata var. fruticosa
-
dwarf ylang ylang, grown in Singapore
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography
Cananga odorata var. fruticosa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Cananga odorata var. fruticosa
-
flower bud
CoTPS2 is highly expressed at the floral bud stage
Cananga odorata var. fruticosa
-
leaf
very low expression level
Cananga odorata var. fruticosa
-
additional information
the enzyme expression increases from undeveloped small flowers to mature green flowers, is absent in mature yellow flowers, but very high in flower buds
Cananga odorata var. fruticosa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2E,6E)-farnesyl diphosphate
-
743000
Cananga odorata var. fruticosa
beta-copaene + diphosphate
-
-
-
?
additional information
enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene
743000
Cananga odorata var. fruticosa
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CoTPS2
-
Cananga odorata var. fruticosa
TPS2
-
Cananga odorata var. fruticosa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Cananga odorata var. fruticosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Cananga odorata var. fruticosa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene TPS2, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, transient and functional recombinant expression of YFP-tagged enzyme CoTPS2 in transgenic Nicotiana benthamiana leaves in cytosol under control of CaMV 35S promoter via Agrobacterium tumefaciens-mediated transfection method
Cananga odorata var. fruticosa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Cananga odorata var. fruticosa
5829
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, enzyme CoTPS2 has the conserved aspartate-rich motif (DDXXD) and NSE/DTE motifs that chelate divalent metal ions, typically Mg2+, in the C-terminal domain
Cananga odorata var. fruticosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2E,6E)-farnesyl diphosphate
Cananga odorata var. fruticosa
-
beta-copaene + diphosphate
-
-
?
additional information
Cananga odorata var. fruticosa
enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography
Cananga odorata var. fruticosa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Cananga odorata var. fruticosa
-
flower bud
CoTPS2 is highly expressed at the floral bud stage
Cananga odorata var. fruticosa
-
leaf
very low expression level
Cananga odorata var. fruticosa
-
additional information
the enzyme expression increases from undeveloped small flowers to mature green flowers, is absent in mature yellow flowers, but very high in flower buds
Cananga odorata var. fruticosa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2E,6E)-farnesyl diphosphate
-
743000
Cananga odorata var. fruticosa
beta-copaene + diphosphate
-
-
-
?
additional information
enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene
743000
Cananga odorata var. fruticosa
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Cananga odorata var. fruticosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Cananga odorata var. fruticosa
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the terpene sythase superfamily, subfamily TPS-a. The arginine-tryptophan motif, R(R)X8W, present at the N terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS2. CoTPS2 does not contain a plastid Tp sequence
Cananga odorata var. fruticosa
metabolism
the enzyme is part of the biosynthesis of volatile organic compounds in flowers
Cananga odorata var. fruticosa
physiological function
recombinant CoTPS2 catalyses the synthesis of three compounds, beta-ylangene, beta-copaene, and beta-cubebene, from farnesyl diphosphate. CoTPS2 is a multifunctional beta-ylangene/beta-copaene/beta-cubebene synthase capable of producing three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene. The enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes. Enzyme CoTPS2 is a multifunctional terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene
Cananga odorata var. fruticosa
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the terpene sythase superfamily, subfamily TPS-a. The arginine-tryptophan motif, R(R)X8W, present at the N terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS2. CoTPS2 does not contain a plastid Tp sequence
Cananga odorata var. fruticosa
metabolism
the enzyme is part of the biosynthesis of volatile organic compounds in flowers
Cananga odorata var. fruticosa
physiological function
recombinant CoTPS2 catalyses the synthesis of three compounds, beta-ylangene, beta-copaene, and beta-cubebene, from farnesyl diphosphate. CoTPS2 is a multifunctional beta-ylangene/beta-copaene/beta-cubebene synthase capable of producing three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene. The enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes. Enzyme CoTPS2 is a multifunctional terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene
Cananga odorata var. fruticosa
Other publictions for EC 4.2.3.127
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743000
Jin
The floral transcriptome of y ...
Cananga odorata var. fruticosa
J. Exp. Bot.
66
3959-3975
2015
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1
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1
1
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1
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1
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4
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2
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2
1
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1
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1
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1
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1
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4
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2
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1
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1
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3
3
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713839
Lopez-Gallego
Selectivity of fungal sesquite ...
Coprinopsis cinerea
Appl. Environ. Microbiol.
76
7723-7733
2010
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1
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5
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3
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2
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1
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1
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2
1
1
1
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1
3
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1
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5
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3
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1
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2
1
1
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1
3
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1
1
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714733
Lopez-Gallego
Sesquiterpene synthases Cop4 a ...
Coprinopsis cinerea
ChemBioChem
11
1093-1106
2010
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2
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1
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1
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1
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1
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1
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705783
Agger
Diversity of sesquiterpene syn ...
Coprinopsis cinerea
Mol. Microbiol.
72
1181-1195
2009
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1
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2
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1
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1
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4
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2
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1
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