Literature summary for 4.2.3.126 extracted from

Jones, C.G.; Keeling, C.I.; Ghisalberti, E.L.; Barbour, E.L.; Plummer, J.A.; Bohlmann, J.
Isolation of cDNAs and functional characterisation of two multi-product terpene synthase enzymes from sandalwood, Santalum album L (2008), Arch. Biochem. Biophys., 477, 121-130.

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Cloned(Commentary)

Cloned (Comment)	Organism
SasesquiTPS1, DNA and amino acid sequence determination and analysis, sequence comparison, functional expression of His6-tagged full-length enzyme in Escherichia coli	Santalum album

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates, required	Santalum album
Mn2+	activates, 10fold less affective compared to Mg2+	Santalum album

Organism

Organism	UniProt	Comment	Textmining
Santalum album	B5A435	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant HIs6-tagged SasesquiTPS1 from Escherichia coli by nickel affinity chromatography	Santalum album

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Santalum album	gamma-muurolene + diphosphate	SasesquiTPS1 with (2E,6E)-farnesyl diphosphate as substrate produces a complex mixture of mono- and bicyclic sesquiterpenes. The main products as analysed by GC-MS and GC-FID are the cyclic alcohol germacrene D-4-ol, and helminthogermacrene. Also present are alpha-bulnesene, gamma-muurolene, alpha- and beta-selinenes, as well as several other minor bicyclic compounds, alpha-humulene and nerolidol are detected in proportions below 5%	?

Subunits

Subunits	Comment	Organism
More	SasesquiTPS1 shows the R(R/P)X8W motif at positions 19-29, and the DDXXD motif	Santalum album

Synonyms

Synonyms	Comment	Organism
SasesquiTPS1	-	Santalum album
sesquiterpene synthase	-	Santalum album

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Release 2023.1 (January 2023)