Cloned (Comment) | Organism |
---|---|
gene DOIS, expression in Escherichia coli strain GI724 from plasmid pGA-btrC | Niallia circulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | introduction of DOIS gene btrC from Bacillus circulans and disruption of genes for phosphoglucose isomerase, D-glucose 6-phosphate dehydrogenase, and phosphoglucomutase, pgi, zwf and pgm, respectively, in Escherichia coli strain GI724. While GI724DELTAP/pGA-btrC and GI724DELTAPP/pGA-btrC possess functional Zwf and thus can catabolize glucose via the pentose phosphate pathway, glucose catabolism is completely inhibited in GI724DELTAPZ/pGA-btrC and GI724DELTAPZP/pGA-btrC due to the deletion of both pgi and zwf genes | Niallia circulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | Niallia circulans | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | Niallia circulans SANK72073 | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q9S5E2 | gene btrC | - |
Niallia circulans SANK72073 | Q9S5E2 | gene btrC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | - |
Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | - |
Niallia circulans SANK72073 | 2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DOIS | - |
Niallia circulans |
General Information | Comment | Organism |
---|---|---|
metabolism | 2-deoxy-scyllo-inosose synthase, DOIS, is a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics | Niallia circulans |