Cloned (Comment) | Organism |
---|---|
genes btrC and btrC2, co-overexpression in Escherichia coli strain JM109 | Niallia circulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption of gene btrC2 by insertion of a tetracycline resistance cassette | Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
D-glucose 6-phosphate | pH 7.7, 35°C, native enzyme | Niallia circulans | |
0.23 | - |
D-glucose 6-phosphate | pH 7.7, 35°C, recombinant wild-type enzyme, BtrC | Niallia circulans | |
0.42 | - |
D-glucose 6-phosphate | pH 7.7, 35°C, recombinant wild-type enzyme, BtrC + BtrC2 | Niallia circulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Niallia circulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | Niallia circulans | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | Niallia circulans SANK72073 | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q9S5E2 | genes btrC and btrC2, the latter encoding the 20 kDa enzyme subunit | - |
Niallia circulans SANK72073 | Q9S5E2 | genes btrC and btrC2, the latter encoding the 20 kDa enzyme subunit | - |
Purification (Comment) | Organism |
---|---|
recombinant BtrC2 from Escherichia coli strain JM109 by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration | Niallia circulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | a butirosin producer culture | Niallia circulans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | - |
Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | - |
Niallia circulans SANK72073 | 2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BtrC2 | - |
Niallia circulans |
DOI synthase | - |
Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Niallia circulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
D-glucose 6-phosphate | pH 7.7, 35°C, native enzyme | Niallia circulans | |
0.44 | - |
D-glucose 6-phosphate | pH 7.7, 35°C, recombinant wild-type enzyme, BtrC + BtrC2 | Niallia circulans | |
1 | - |
D-glucose 6-phosphate | pH 7.7, 35°C, recombinant wild-type enzyme, BtrC | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Niallia circulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Niallia circulans |
General Information | Comment | Organism |
---|---|---|
malfunction | disruption of gene btrC2 reduces the growth rate and antibiotics production, the growth rate is restored by addition of NH4Cl, both by addition of yeast extract | Niallia circulans |
metabolism | key enzyme in the biosynthesis of 2-deoxytstreptamine. The function od BtrC2 in biosynthesis of butirosin is indirect. BtrC2 is involved in secondary as well as primary metabolism probably playing a role in stabilizing and regulating DOI synthase | Niallia circulans |