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Literature summary for 4.2.3.124 extracted from

  • Tamegai, H.; Nango, E.; Koike-Takeshita, A.; Kudo, F.; Kakinuma, K.
    Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans (2002), Biosci. Biotechnol. Biochem., 66, 1538-1545.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes btrC and btrC2, co-overexpression in Escherichia coli strain JM109 Niallia circulans

Protein Variants

Protein Variants Comment Organism
additional information disruption of gene btrC2 by insertion of a tetracycline resistance cassette Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
D-glucose 6-phosphate pH 7.7, 35°C, native enzyme Niallia circulans
0.23
-
D-glucose 6-phosphate pH 7.7, 35°C, recombinant wild-type enzyme, BtrC Niallia circulans
0.42
-
D-glucose 6-phosphate pH 7.7, 35°C, recombinant wild-type enzyme, BtrC + BtrC2 Niallia circulans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Niallia circulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate Niallia circulans
-
2-deoxy-L-scyllo-inosose + phosphate
-
?
D-glucose 6-phosphate Niallia circulans SANK72073
-
2-deoxy-L-scyllo-inosose + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Niallia circulans Q9S5E2 genes btrC and btrC2, the latter encoding the 20 kDa enzyme subunit
-
Niallia circulans SANK72073 Q9S5E2 genes btrC and btrC2, the latter encoding the 20 kDa enzyme subunit
-

Purification (Commentary)

Purification (Comment) Organism
recombinant BtrC2 from Escherichia coli strain JM109 by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration Niallia circulans

Source Tissue

Source Tissue Comment Organism Textmining
additional information a butirosin producer culture Niallia circulans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate
-
Niallia circulans 2-deoxy-L-scyllo-inosose + phosphate
-
?
D-glucose 6-phosphate
-
Niallia circulans SANK72073 2-deoxy-L-scyllo-inosose + phosphate
-
?

Synonyms

Synonyms Comment Organism
BtrC2
-
Niallia circulans
DOI synthase
-
Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.073
-
D-glucose 6-phosphate pH 7.7, 35°C, native enzyme Niallia circulans
0.44
-
D-glucose 6-phosphate pH 7.7, 35°C, recombinant wild-type enzyme, BtrC + BtrC2 Niallia circulans
1
-
D-glucose 6-phosphate pH 7.7, 35°C, recombinant wild-type enzyme, BtrC Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
assay at Niallia circulans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Niallia circulans

General Information

General Information Comment Organism
malfunction disruption of gene btrC2 reduces the growth rate and antibiotics production, the growth rate is restored by addition of NH4Cl, both by addition of yeast extract Niallia circulans
metabolism key enzyme in the biosynthesis of 2-deoxytstreptamine. The function od BtrC2 in biosynthesis of butirosin is indirect. BtrC2 is involved in secondary as well as primary metabolism probably playing a role in stabilizing and regulating DOI synthase Niallia circulans