Literature summary for 4.2.3.123 extracted from

Ker, D.S.; Pang, S.L.; Othman, N.F.; Kumaran, S.; Tan, E.F.; Krishnan, T.; Chan, K.G.; Othman, R.; Hassan, M.; Ng, C.L.
Purification and biochemical characterization of recombinant Persicaria minor beta-sesquiphellandrene synthase (2017), PeerJ, 5, e2961 .

Search for more literature for 4.2.3.123

Cloned(Commentary)

Cloned (Comment)	Organism
sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged and N-terminally truncated enzyme PmSTS_DELTA124 in Escherichia coli strain BL21(DE3)	Persicaria minor

Protein Variants

Protein Variants	Comment	Organism
additional information	N-terminal truncation, resulting in mutant PmSTS_DELTA124, does not affect the enzyme activity of PmSTS	Persicaria minor

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten steady-state kinetics	Persicaria minor
0.0102	-	(2E,6E)-farnesyl diphosphate	pH 8.0, 30°C, recombinant enzyme mutant PmSTS_DELTA124	Persicaria minor

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	enzyme PmSTS contains an altered conserved metal binding motif	Persicaria minor

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65000	-	gel filtration	Persicaria minor

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	Persicaria minor	-	beta-sesquiphellandrene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Persicaria minor	I6XZ73	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme PmSTS from Escherichia coli strain BL21(DE3), the recombinant enzyme has an N-terminal truncation, that dramatically improves the homogeneity of PmSTS during protein purification, suggesting that the disordered N-terminal region may have caused the formation of soluble aggregate	Persicaria minor

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Persicaria minor	-
stem	-	Persicaria minor	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Persicaria minor	beta-sesquiphellandrene + diphosphate	-	?
additional information	the recombinant purified enzyme catalyses the synthesis of beta-sesquiphellandrene, not alpha-farnesene, GC-MS analysis of enzyme reaction products, overview. The major product is beta-sesquiphellandrene (KI:1516, about 97%) and beta-farnesene (about 3%) as a minor product	Persicaria minor	?	-	?

Synonyms

Synonyms	Comment	Organism
PmSTS	-	Persicaria minor

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	recombinant enzyme	Persicaria minor

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	45	and below, activity range, profile overview. At 40°C, the enzymatic activity is less than 40% compared to that at 30°C, inactivation at 50°C	Persicaria minor

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.078	-	(2E,6E)-farnesyl diphosphate	pH 8.0, 30°C, recombinant enzyme mutant PmSTS_DELTA124	Persicaria minor

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	recombinant enzyme	Persicaria minor

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	activity range, profile overview	Persicaria minor

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the sesquiterpene synthases, it contains the NSE/DTE motif commonly found in terpene synthases	Persicaria minor
additional information	homology model of PmSTS shows the structure domain and active site of the enzyme using the structures of monoterpene synthase Salvia officinalis (+)-bornyl diphosphate synthase (PDB ID 1N20) and Mentha spicata 4S-limonene synthase (PDB ID 2ONG), and sesquiterpene Nicotiana tabacum 5-epi-aristolochene synthase (PDB ID 3M01), Gossypium arboreum beta-cadinene synthase (PDB ID 3G4F) and Artemisia annua alpha-bisabolol synthase (PDB ID 4FJQ). The homology structure of PmSTS that allows good fitting of a substrate analogue into the catalytic active site suggests that PmSTS may adopt a sesquiterpene biosynthesis mechanism similar to other plant sesquiterpene synthases	Persicaria minor

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
7.65	-	(2E,6E)-farnesyl diphosphate	pH 8.0, 30°C, recombinant enzyme mutant PmSTS_DELTA124	Persicaria minor

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Release 2023.1 (January 2023)