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Literature summary for 4.2.3.121 extracted from
- Characterization of a monoterpene synthase from Paeonia lactiflora producing alpha-pinene as its single product (2016), Biotechnol. Lett., 38, 1213-1219 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| enzyme PlPIN, cloned from roots, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli strain Transetta (DE3) | Paeonia lactiflora |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Paeonia lactiflora |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate | Paeonia lactiflora | - |
alpha-pinene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paeonia lactiflora | A0A0G3BIB7 | i.e. Paeonia albiflora | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate | - |
Paeonia lactiflora | alpha-pinene + diphosphate | - |
? | |
| additional information | GC-MS analysis reaction product analysis. Enzyme PlPIN employs geranyl diphosphate as a specific substrate to produce alpha-pinene as a unique product | Paeonia lactiflora | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha pinene sythase | - |
Paeonia lactiflora |
| alpha-pinene sythase | - |
Paeonia lactiflora |
| More | cf. EC 4.2.3.119 | Paeonia lactiflora |
| PlPIN | - |
Paeonia lactiflora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Paeonia lactiflora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Paeonia lactiflora |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the TPS enzyme superfamily, clade TPS-b, where it is clustered with monoterpene synthases | Paeonia lactiflora |
| physiological function | the terpene synthase PlPIN from Paeonia lactiflora catalyzes the conversion of geranyl pyrophosphate to alpha-pinene and is involved in the biosynthesis of paeoniflorin | Paeonia lactiflora |
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Release 2023.1 (January 2023)
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