Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pinus taeda |
Crystallization (Comment) | Organism |
---|---|
modeling of structure against the tobacco sesquiterpene synthase 5-epi-aristolochene synthase and the monoterpene synthase bornyl diphosphate synthase from common sage | Pinus taeda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
geranyl diphosphate | pH 6.5, 31°C | Pinus taeda |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required for optimum activity, NH4+ may substitute | Pinus taeda | |
Mg2+ | or Mn2+, required. 67% of reaction velocity with Mn2+ | Pinus taeda | |
Mn2+ | or Mg2+, required. Mn2+ is preferred | Pinus taeda | |
NH4+ | may substitute for K+ for optimum activity | Pinus taeda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64500 | - |
x * 64500, SDS-PAGE of recombinant protein lacking transit peptide | Pinus taeda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus taeda | Q84KL3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a putative transit peptide | Pinus taeda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Pinus taeda | (+)-alpha-pinene + diphosphate | products are 97% (+)-alpha-pinene, 3% (-)-alpha-pinene | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64500, SDS-PAGE of recombinant protein lacking transit peptide | Pinus taeda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pinus taeda |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
half-maximal reaction velocity | Pinus taeda |
7.5 | - |
half-maximal reaction velocity | Pinus taeda |