Literature summary for 4.2.3.121 extracted from

Phillips, M.A.; Wildung, M.R.; Williams, D.C.; Hyatt, D.C.; Croteau, R.
cDNA isolation, functional expression, and characterization of (+)-alpha-pinene synthase and (-)-alpha-pinene synthase from loblolly pine (Pinus taeda): stereocontrol in pinene biosynthesis (2003), Arch. Biochem. Biophys., 411, 267-276.

Search for more literature for 4.2.3.121

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pinus taeda

Crystallization (Commentary)

Crystallization (Comment)	Organism
modeling of structure against the tobacco sesquiterpene synthase 5-epi-aristolochene synthase and the monoterpene synthase bornyl diphosphate synthase from common sage	Pinus taeda

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.047	-	geranyl diphosphate	pH 6.5, 31°C	Pinus taeda

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	required for optimum activity, NH4+ may substitute	Pinus taeda
Mg2+	or Mn2+, required. 67% of reaction velocity with Mn2+	Pinus taeda
Mn2+	or Mg2+, required. Mn2+ is preferred	Pinus taeda
NH4+	may substitute for K+ for optimum activity	Pinus taeda

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64500	-	x * 64500, SDS-PAGE of recombinant protein lacking transit peptide	Pinus taeda

Organism

Organism	UniProt	Comment	Textmining
Pinus taeda	Q84KL3	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	sequence contains a putative transit peptide	Pinus taeda

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate	-	Pinus taeda	(+)-alpha-pinene + diphosphate	products are 97% (+)-alpha-pinene, 3% (-)-alpha-pinene	?

Subunits

Subunits	Comment	Organism
?	x * 64500, SDS-PAGE of recombinant protein lacking transit peptide	Pinus taeda

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Pinus taeda

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	-	half-maximal reaction velocity	Pinus taeda
7.5	-	half-maximal reaction velocity	Pinus taeda

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Release 2023.1 (January 2023)