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Literature summary for 4.2.3.121 extracted from

  • Wheeler, C.J.; Croteau, R.
    Terpene cyclase catalysis in organic solvent/minimal water media: demonstration and optimization of (+)-alpha-pinene cyclase activity (1986), Arch. Biochem. Biophys., 248, 429-434.
    View publication on PubMed
Search for more literature for 4.2.3.121

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Salvia officinalis

Organic Solvent Stability

Organic Solvent Comment Organism
diisopropyl ether enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene Salvia officinalis
hexadecane enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene Salvia officinalis
hexane enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene. Reactions run in hexane generate product at roughly one-third the rate of companion assays in which the lyophilized protein is rehydrated in distilled water. Relative proportion of alpha-pinene in product distribution is optimal in presence of 5-10% water Salvia officinalis
additional information enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene Salvia officinalis

Organism

Organism UniProt Comment Textmining
Salvia officinalis
-
-
-

Storage Stability

Storage Stability Organism
lyophilization and rehydration in destilled water results in loss of about 10% of activity Salvia officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate
-
 Salvia officinalis (+)-alpha-pinene + diphosphate products are 25% (+)-alpha-pinene, 25% (+)-camphene, 43% (+)-limonene, and 7% myrcene. Reaction in organic solvent gives the same olefin distribution as in aqueous buffer ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
31
-
-
 Salvia officinalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 55% of maximum activity Salvia officinalis
58
-
 20% of maximum activity Salvia officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 aqueous assay Salvia officinalis
6.2
-
 assay in hexane Salvia officinalis